転写因子Ifh1は,酵母リボソームタンパク質遺伝子の重要な調節因子です

Joseph T Wade¹, Daniel B Hall, Kevin Struhl

¹Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Nature

|December 24, 2004

まとめ

リボソームタンパク質 (RP) の遺伝子転写は,細胞成長の鍵です. この研究は,Ifh1を特定の調節体として特定し,特に環境ストレス下では,RP遺伝子発現を調整するために不可欠である.

科学分野:

分子生物学は分子生物学である.
遺伝子規制遺伝子規制
細胞プロセス細胞のプロセス

背景:

リボソームタンパク質 (RP) 遺伝子は,タンパク質合成と細胞成長に不可欠です.
それらの調整された調節は,生理学的条件に細胞容量を調整するために不可欠です.
RNAポリメラーゼIIの転写の約50%がRP遺伝子に割り当てられています.

研究の目的:

RP遺伝子発現を制御する特定の転写レギュレータを特定するために.
RP遺伝子の座標調節メカニズムを解明する.
RP遺伝子調節における転写因子Fhl1とIfh1の役割を理解する.

主な方法:

酵母モデルを使用してRPプロモーターと転写因子の関連性を調査しました.
Rap1とDNA要素に対する因子関連の依存度を分析した.
環境ストレスが因子関連と転写レベルに及ぼす影響を調べました.

主要な成果:

Fhl1 と Ifh1 は,ほぼ独占的に RP プロモーターと連携し,Rap1 に依存しています.
Ifh1は,Fhl1のフォークヘッド関連ドメイン (FHA) を介して採用されます.
環境ストレスがIfh1関連性を著しく低下させ,RP遺伝子転写に影響を及ぼします.

結論:

RPプロモーターとのIfh1関連は,それらの座標表現のための重要な規制ステップです.
このメカニズムにより,細胞は生理的な要求やストレスに反応して,タンパク質合成を微調整することができます.

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