SCFCdc4によるFus3調節されたTec1分解は,酵母における交配中のMAPKシグナル伝達特異性を決定する

Song Chou¹, Lan Huang, Haoping Liu

¹Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA.

Cell

|December 29, 2004

まとめ

Fus3キナーゼは,Tec1タンパク質を分解対象にすることで,交配中の酵母線維を防止します. この特殊なメカニズムは,異なる環境信号に対する適切な細胞応答を保証します.

科学分野:

細胞生物学細胞生物学
分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー

背景:

ミトゲン活性化タンパク質キナーゼ (MAPK) カスケードは,細胞の成長と分化を調節する.
酵母Saccharomyces cerevisiaeでは,異なるMAPK経路が交配とフィラメント化を制御しています.
Fus3 MAPKは,交配時にフィラメントを防止する必要がありますが,そのメカニズムは不明です.

研究の目的:

フェロモンの反応中にFus3がフィラメントプログラムの活性化を防ぐ方法を解明する.
Fus3媒介によるフィラメンテーションの阻害に関与する分子プレーヤーを特定するために.

主な方法:

フェロモン反応中のフィラメンテーションコファクターであるTec1の分解を調査した.
Tec1.1.のFus3媒介ユビキチン化および分解を研究するために,in vitroおよびin vivoアッセイを使用しました.
T273におけるTec1のリン酸化が,そのユビキチン化と分解における役割を調査した.

主要な成果:

線維基因発現のコファクターであるTec1は,フェロモン反応中に急速に分解されます.
Fus3キナーゼは,SCF-Cdc4ユビキチンリガゼを介して,Tec1のユビキチン化と分解を直接誘導する.
Fus3によるTec1のスレオニン273でのリン酸化は,そのユビキチン化と分解に不可欠である.

結論:

Fus3は,Tec1コファクターの分解を促進することによって,交配中に糸状の成長を阻害する.
この分解は,Tec1のFus3依存型リン酸化によって媒介され,それをユビキチン化およびプロテアソーム破壊に標的とする.
これは,並列のMAPK経路におけるシグナリング特異性を確保するための重要なメカニズムを提供します.

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