関連する実験動画
Updated: May 5, 2026

04:36
Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
14.7K
Tec1転写因子のフェロモン依存破壊は,酵母におけるMAPキナーゼシグナル伝達特異性のために必要である
Marie Z Bao1, Monica A Schwartz, Greg T Cantin
1Department of Biochemistry and Biophysics, University of California, 600 16th Street, San Francisco, CA 94143, USA.
Cell
|December 29, 2004
まとめ
酵母細胞はFus3を使用してTec1タンパク質を分解し,交配信号が糸状の成長を活性化するのを防ぐ. これは,標的型タンパク質破壊を通じて,異なる細胞経路機能を保証します.
科学分野:
- 細胞の信号伝達経路は,
- 遺伝子調節の分子メカニズム
- イーストの遺伝学と細胞生物学
背景:
- 交配のための酵母MAPK経路と有糸成長経路は,同じ成分を持っていますが,結果は異なります.
- 交配特有のMAPKであるFus3は,通常,未知のメカニズムによって経路の交差を防ぐ.
研究 の 目的:
- Fus3が酵母交配と糸状成長経路の交差を防ぐメカニズムを解明する.
- 信号特異性の維持に関与する分子プレーヤーを特定する.
主な方法:
- 研究されたフィラメント転写因子のFus3依存性分解,Tec1.
- 遺伝子削除や点変異 (TEC1 T273Aなど) を含む酵母遺伝子を活用した.
- タンパク質とタンパク質の相互作用と,Fus3とTec1.1を含む in vitro キナーゼアッセイを調査した.
- SCFのユビキチン・リガゼ成分 (Dia2,Cdc53) がTec1の分解における役割を評価した.
主要な成果:
- フェロモンのシグナル伝達により,Tec1.1.のFus3依存性分解が誘発されます.
- Tec1の分解には,Fus3キナーゼ活性とThreonine 273.3でのリン酸化が必要です.
- Fus3はT273.3でTec1を直接リン酸化する.
- Tec1の破壊は,SCFのユビキチンリガゼ複合体によって媒介され,Dia2とCdc53.3が関与しています.
- Tec1のリン酸化やFus3/Dia2の機能を妨げる突然変異は,交尾中にフィラメント化遺伝子を活性化させ,シグナリング特異性の喪失につながります.
結論:
- Fus3による競合する経路の転写因子 (Tec1) の信号誘発破壊は,酵母MAPK経路の特異性を確保するための重要なメカニズムです.
- このプロセスは,交配中に糸状成長遺伝子の不適切な活性化を防止します.
関連する概念動画
Yeast Signaling
15.7K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
15.7K
Eukaryotic Transcription Inhibitors
9.1K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.1K
Microtubule Associated Proteins (MAPs)
4.8K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
4.8K
Interactions Between Signaling Pathways
4.7K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
4.7K
MAPK Signaling Cascades
7.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
PI3K/mTOR/AKT Signaling Pathway
5.1K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.1K

