Maxの代替形態は,Myc-rasのコトランスフォーメーションの強化剤または抑制剤として作用する
T P Mäkelä1, P J Koskinen, I Västrik
1Department of Virology, University of Helsinki, Finland.
Science (New York, N.Y.)
|April 17, 1992
まとめ
max遺伝子は,c-Myc機能の陰性および陽性レギュレータの両方として作用できるタンパク質をコードします. 切断された形であるデルタマックスは,全長マックスタンパク質とは異なり,細胞の変容を促進します.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- タンパク質の機能 タンパク質の機能
背景:
- Maxは,Mycタンパク質とDNA結合複合体を形成する基本的なヘリックス・ループ・ヘリックス・ルシンのジッパータンパク質です.
- c-Mycは,細胞の成長と増殖に関与する重要な転写因子です.
研究 の 目的:
- "デルタマックス"と呼ばれる,新たに識別された,代替的にスプライスされたマックスの形態の機能を調査する.
- c-Myc活性と細胞変容の調節における全長マックスとデルタマックスの役割を決定する.
主な方法:
- 代替的にスプライスされたmRNAのエンコーディングデルタの識別と特徴 Max.
- c-Myc.によるMaxとdelta MaxのDNA結合能力の分析
- ネズミの胚性線維芽細胞におけるミクロラスのコトランスフォーメーションアッセイを用いた変容の可能性の評価.
主要な成果:
- デルタマックスは,CACGTGモチーフをc-Mycと結合する能力を保持しているが,核の局所化と規制ドメインが欠けている.
- 試験中の全長マックス抑制細胞変容.
- デルタマックスは,ミクロラスのコトランスフォーメーションアッセイで細胞変換を強化した.
結論:
- マックス遺伝子は,c-Myc機能の陰性 (Max) と陽性 (delta Max) のレギュレータの両方を生成することができます.
- Maxの代替スプライシングは,細胞変容における対極的な役割を持つ機能的に異なるタンパク質を生成します.
- これらの発見は,c-Myc媒介の細胞プロセスにおけるマックスの複雑な調節作用を強調しています.
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