WntとFGF受容体を敵対させる:内部からの敵 (ER)

Xi He¹

¹Division of Neuroscience, Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA.

Cell

|February 1, 2005

まとめ

新しい分子であるShisaは,エンドプラズマ網膜内のFrizzledおよび線維細胞成長因子受容体の成熟を阻害する. この作用はWntとFGFのシグナル伝達を阻害し,Xenopusの発達における前部パターンを促進する.

科学分野:

発達生物学発達生物学について
分子生物学は分子生物学である.
細胞生物学細胞生物学

背景:

Frizzled (Fz) とFibroblast成長因子受容体 (FGFRs) は,胚の発達に不可欠である.
WntとFGFの信号伝達経路は,重要な発達過程を調節する.
適切な受容体の成熟は,信号伝導に不可欠である.

研究の目的:

WntとFGFのシグナル伝達経路の新しいレギュレータを特定する.
新しく発見された分子"シサ"の機能を明らかにするために.
クセノプスの前部パターニングの基礎となる分子機構を理解する.

主な方法:

クセノプスの胚におけるシサ遺伝子の発現分析.
FzとFGFRとのShisaの相互作用を決定する生化学的分析.
ShisaのノックダウンまたはXenopus.の過剰表現を含む機能的研究.

主要な成果:

ヤマモト et al. ほか Shisaを新型エンドプラズマ網膜 (ER) 局所化されたタンパク質として特定しました.
Shisaは,Frizzled (Fz) と線維細胞成長因子受容体 (FGFRs) の成熟を抑制することが判明しました.
シーサは,WntとFGFの信号伝達の細胞自律的アンタゴニストとして作用する.

結論:

シーサは,受容体の成熟を防ぐことによって,WntとFGFの信号伝達を逆転させる上で重要な役割を果たしています.
シーサの機能は,クセノプスの発達中の前面のパターンを促進するために不可欠です.
シーサの発見は,受容体チロシンキナーゼとサーペンタイン受容体シグナル伝達の調節に関する新しい洞察を提供します.

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