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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

7.0K
Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent...
7.0K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

7.5K
This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator...
7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

18.9K
This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this...
18.9K
Generation of Genetically Modified Mice through the Microinjection of Oocytes10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

21.6K
The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping...
21.6K
DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

21.0K
RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in...
21.0K
Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans10:58

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

18.2K
We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C.
18.2K

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Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

7.0K

ラマン・インジェクションレーザーのレーザーです.

Mariano Troccoli1, Alexey Belyanin, Federico Capasso

  • 1Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA. troccoli@deas.harvard.edu

Nature
|February 25, 2005
PubMed
まとめ
この要約は機械生成です。

研究者らは,半導体注入型ラマンレーザーの新型を開発した. この電気駆動装置は,外部ポンプの必要性を排除し,コンパクトな赤外線光源のラマン増益を大幅に高めます.

さらに関連する動画

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

18.9K

関連する実験動画

Last Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

7.0K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

18.9K

科学分野:

  • 非線形光学は,非線形光学である.
  • 半導体レーザー 半導体レーザー
  • 赤外線フォトニクスとは

背景:

  • 刺激ラーマン散射 (SRS) は調節可能な光源を可能にしますが,通常は強力な外部レーザーポンプを必要とします.
  • 既存のラーマンレーザーは,利得が低く,実用的な応用が限られている.
  • 量子カスケードレーザー (QCL) は,中赤外線の電気ポンプと光学増益を提供します.

研究 の 目的:

  • 半導体注入ラーマンレーザーを開発し,従来のラーマン源の限界を克服する.
  • 外部光学ポンプなしで高いラーマン増益と効率を達成するために.
  • コンパクトで波長が敏捷な中・遠赤外線光源を作成する.

主な方法:

  • 量子カスケードレーザーのアクティブ領域内で,トリプル共振刺激ラーマン散乱を利用した.
  • 量子カスケードレーザーを内部光学ポンプとして統合し,電気運転を可能にしました.
  • 外部レーザーポンプなしで動作する半導体注入ラーマンレーザーを実証しました.

主要な成果:

  • 従来のラーマンレーザーと比較して,ラーマン増益で数桁の増幅を達成しました.
  • 高い変換効率とレーザー操作のための低い値を示しています.
  • 電気駆動型,コンパクトなラーマンレーザーソースを成功裏に実現しました.

結論:

  • 開発された半導体注入ラーマンレーザーは,既存の技術に比べて著しく進歩しています.
  • この装置は,外部のレーザーポンプの必要性を回避し,操作を簡素化し,サイズを小さくします.
  • 新しい世代のコンパクトで,調節可能で,効率的な中,遠赤外線半導体光源の道を開く.