植物病原性細菌エフェクタータンパク質をエウカリオットサイクロフィリンによって活性化させる
Gitta Coaker1, Arnold Falick, Brian Staskawicz
1Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
Science (New York, N.Y.)
|March 5, 2005
まとめ
植物免疫受容体RPS2は,防御を誘発するためにバクテリアのエフェクタータンパク質,AvrRpt2を使用します. サイクロフィリンによって活性化されたAvrRpt2はRIN4を裂き,Pseudomonas syringaeに対する植物耐性を開始します.
科学分野:
- 植物免疫 植物免疫とは
- 分子植物-微生物の相互作用
- バクテリアの病原体発生です.
背景:
- 高級植物には,バクテリアのエフェクタータンパク質を検出する天生的免疫力があります.
- アラビドプシスのPseudomonas syringaeに対する耐性は,AvrRpt2のような効果因子が存在すると,RPS2タンパク質に依存する.
研究 の 目的:
- AvrRpt2がRPS2媒介の植物免疫を活性化するメカニズムを解明する.
- AvrRpt2の活性化に起因する宿主因子を特定する.
主な方法:
- 植物防衛シグナル伝達におけるAvrRpt2の役割を調査した.
- AvrRpt2のプロテアゼ活性を活性化する宿主因子を特定しました.
- 植物細胞内のAvrRpt2の標的を決定しました.
主要な成果:
- AvrRpt2は,アラビドプシスのタンパク質RIN4.4を分割するシステインプロテアゼです.
- AvrRpt2によるRIN4割れは,RPS2媒介による抵抗の信号として作用する.
- AvrRpt2の活性化と,その後のRIN4の分裂には,サイクロフィリンとして知られるエウカリオット因子が必要である.
結論:
- サイクロフィリン媒介によるAvrRpt2の活性化は,そのタンパク質酶活性に不可欠である.
- アクティブのAvrRpt2はRIN4を直接分裂させ,植物防衛反応を誘発する.
- この経路は,細菌病原体に対する洗練された植物監視メカニズムを強調しています.
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