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Updated: Jul 23, 2026

Synthesis of an Intein-mediated Artificial Protein Hydrogel
15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

小分子リガンドで抑制できるインテインの設計

Steffen Brenzel1, Henning D Mootz

  • 1Philipps-Universität Marburg, Fachbereich Chemie/Biochemie, Hans-Meerwein-Strasse, 35032 Marburg, Germany.

Journal of the American Chemical Society
|March 24, 2005
PubMed
まとめ

研究者は,分割インテインを使用して制御可能なタンパク質スプライシングシステムを設計しました. この新しいインテインは,小分子リガンドを使用して"オフ"することができ,翻訳後のタンパク質機能に対する正確な制御を提供します.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...