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関連する概念動画

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Structure of a Gene01:30

Structure of a Gene

A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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関連する実験動画

Updated: Jul 9, 2026

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
14:53

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Published on: May 14, 2014

単細胞レベルでの遺伝子調節

Nitzan Rosenfeld1, Jonathan W Young, Uri Alon

  • 1Departments of Molecular Cell Biology and Physics of Complex Systems, Weizmann Institute of Science, Rehovot, 76100, Israel.

Science (New York, N.Y.)
|March 26, 2005
PubMed
まとめ

遺伝子調節機能 (GRF) は単細胞で変動し,遺伝回路の精度を制限する. このダイナミックなGRFは,生化学的パラメータと細胞状態の影響を受け,生物学的システムにおける信号伝送に影響を与えます.

科学分野:

  • 分子生物学は分子生物学である.
  • システム生物学 システム生物学
  • 遺伝学 遺伝学とは

背景:

  • 転写因子レベルと遺伝子発現の間の定量的な関係は,遺伝ネットワークにとって根本的なものです.
  • この関係を理解することは,自然と合成の遺伝子回路設計の両方にとって非常に重要です.

研究 の 目的:

  • 個々の生きている細胞における遺伝子調節機能 (GRF) の動的性質を調査する.
  • GRFの変動に寄与する要因と,その影響が信号伝送の精度に与える影響を決定する.
  • 新しい技術を使用して分子単位で生体生化学的パラメータをin vivoで校正する.

主な方法:

  • 光レポーター遺伝子と融合タンパク質を用いたEscherichia coliにおけるバクテリオファージのラムダプロモーターP (R) の特徴化.
  • タンパク質分割における二項式エラーに基づく新しい技術の開発と応用. in vivoパラメータ校正のための.
  • 細胞サイクル時間スケールにおけるタンパク質生産率と内在的なノイズの分析.

主要な成果:

  • 遺伝子調節機能 (GRF) は,個々の生体細胞で動的に変動することが判明しました.
  • タンパク質の生産率は,約1つの細胞周期の時間スケールで変動を示します.

さらに関連する動画

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

関連する実験動画

Last Updated: Jul 9, 2026

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
14:53

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Published on: May 14, 2014

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

  • 固有のノイズは急速に衰退し,生化学的パラメータ,ノイズ,そしてゆっくりと変化する細胞状態は,集団的に効果的な単細胞GRFを定義します.

    • 結論:

    • GRFのダイナミックな変動は,転写遺伝回路における信号伝達の精度を制限する.
    • この発見は,天然の遺伝子回路の定量モデリングの基礎となる.
    • 結果は,性能を改善した合成遺伝回路の合理的な設計のための洞察を提供します.