人間のMpp11 Jタンパク質:リボソームに結合した分子チャペロンは至るところに存在する
Heather A Hundley1, William Walter, Shawn Bairstow
1Department of Biochemistry, 433 Babcock Drive, University of Wisconsin-Madison, Madison, WI 53706, USA.
Science (New York, N.Y.)
|April 2, 2005
まとめ
人間のMpp11のようなリボソーム関連分子チャペロンは,タンパク質の翻訳と折り畳みを結びつける. 酵母ZuoのオートログであるMpp11は,新生ポリペプチド鎖を支援するために,Hsp70のチャペロンと提携しています.
科学分野:
- 分子生物学は分子生物学である.
- タンパク質の折りたたみ
- 細胞メカニズム 細胞メカニズム
背景:
- 特殊な分子チャペロンは,微生物のリボソームと直接相互作用する.
- これらのチャペロンは,脱出するポリペプチドと結合し,トランスレーションとタンパク質の折り畳みを結びつける.
- リボソーム関連チャペロンは,細胞タンパク質ホメオスタシスにとって極めて重要です.
研究 の 目的:
- ユカリオットにおけるリボソーム関連分子チャペロンの進化的保存を調査する.
- 酵母Zuoのヒトのオートロログを特徴づけるために,Mpp11と名付けました.
- Mpp11とHsp70のシャペロンとの相互作用を理解するために.
主な方法:
- 酵母Zuo.の真核オートログを識別するための比較ゲノミクス.
- 酵母で表したヒトMpp11の機能分析.
- シャパロン相互作用を研究するための生化学的分析.
主要な成果:
- Mpp11は酵母Zuoのヒトの正体であり,真核生物の進化を通して維持されてきた.
- 発現したMpp11は酵母でZuo関数を部分的に置換した.
- Mpp11は酵母でSsa (酵母Hsc70同型) と提携しました.
結論:
- リボソーム関連分子チャペロンは,進化的にユカリオットに保存されています.
- Mpp11は,メタゾーンにおけるリボソーム関連チャペロンである.
- Mpp11はおそらくHsc70をメタゾーンにおけるリボソームから出ている新生ポリペプチドに勧誘する.
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