エピモルフィン (epimorphin):エピテリアモルフォゲネシスに不可欠なメゼンキマタンパク質
Y Hirai1, K Takebe, M Takashina
1Biomaterial Research Institute, Yokohama, Japan.
Cell
|May 1, 1992
まとめ
新しく発見されたタンパク質であるエピモルフィン (epimorphin) は,上皮質の形態発生に不可欠である. この細胞表面分子は,胚の発達中に必須の表皮細胞-メゼンキマ相互作用を媒介する.
科学分野:
- 発達生物学 発達生物学とは
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- 表皮細胞形態生成は,表皮細胞とメゼンキマ細胞の連携した相互作用を含む複雑なプロセスです.
- これらの相互作用を制御する分子機構を理解することは,発達生物学と再生医療にとって不可欠です.
研究 の 目的:
- エピテリア・メゼンキマ相互作用に関与する新しい分子を特定し,特徴づけること.
- 新しく特定されたタンパク質が,上皮質の形質変異を調節する役割を明らかにすること.
主な方法:
- ネズミの胚性メゼンキマ細胞からの新しい150 kDaの細胞表面タンパク質の識別と特徴付け.
- 臓器培養におけるタンパク質機能を抑制するモノクローナル抗体の生成.
- cDNAのシーケンシングとNIH 3T3細胞へのトランスフェクション.
- 肺上皮細胞とNIH 3T3細胞を移植した共同培養実験.
主要な成果:
- 新しい150 kDaのタンパク質であるエピモルフィンは,メゼンキマ細胞表面で特定されました.
- エピモーリンに対するモノクローナル抗体は,毛の成長を阻害し,肺上皮質の管状形成を in vitro で阻害した.
- NIH 3T3細胞をエピモルフィンcDNAで感染させると,タンパク質の表面発現が起こりました.
- エピモルフィンを発現するNIH 3T3細胞との共同培養により,正常な肺上皮質管状形態変異が回復しました.
結論:
- エピモルフィン (epimorphin) は,上皮細胞とメゼンキマ細胞の相互作用の重要な媒介体である.
- このタンパク質は,胚の発達中の上皮質の形態変異を調節する上で重要な役割を果たします.
- エピモルフィンは,発達過程を理解し,操作するための潜在的なターゲットです.
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