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The DNA Helix01:16

The DNA Helix

Overview
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...

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Updated: Jul 6, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

DNAを水解する自己抗体.

A M Shuster1, G V Gololobov, O A Kvashuk

  • 1V.A. Engelhardt Institute of Molecular Biology, Academy of Sciences of Russia, Moscow.

Science (New York, N.Y.)
|May 1, 1992
PubMed
まとめ

自身免疫疾患の患者の自己抗体は,DNAを切り離す活性を示しています. 免疫グロブリンM (IgM) と免疫グロブリンG (IgG) と識別されるこのDNAを水解する活動は,標準のデオキシリボヌクレアゼ (DNase) と異なる.

科学分野:

  • 免疫学 免疫学とは
  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー

背景:

  • 自己免疫病理を持つ患者は,独特の血清活動を示すことができます.
  • オートアンチボディは,自己免疫疾患において重要な役割を果たします.
  • オートアンチボディの酵素活性が完全に特徴づけられていない.

研究 の 目的:

  • 自己免疫疾患を有する患者の血清におけるDNAニッキング活動の存在と性質を調査する.
  • DNAの水解に起因する自己抗体を特徴付けるため.
  • 既知のデオキシリボヌクレアゼ (DNase) とのDNA分解パターンを比較する.

主な方法:

  • 自身免疫病理を持つ患者の血清サンプルをDNAニッキング活動のために分析した.
  • 浄化には,アフィニティと高性能液体染色法を使用した.
  • 免疫グロブリンM (IgM) と免疫グロブリンG (IgG) が活性成分として特定されました.
  • 酸性ショックの安定性とDNAの劣化パターンを評価した.

主要な成果:

  • 患者さんの血清にDNAニッキング活性が検出され,これは自己抗体に起因している.

さらに関連する動画

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

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Extraction of Diatom DNA from Water Samples and Tissues
04:20

Extraction of Diatom DNA from Water Samples and Tissues

Published on: November 10, 2023

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Last Updated: Jul 6, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
09:25

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

Extraction of Diatom DNA from Water Samples and Tissues
04:20

Extraction of Diatom DNA from Water Samples and Tissues

Published on: November 10, 2023

  • 浄化された活動は,抗ヒトIgG抗体と反応するIgMとIgGに対応した.
  • これらのDNAを水解する自己抗体は,酸性ショックに対する安定性を示した.
  • これらの自己抗体によって生成されるDNA分解パターンは,DNase Iと血液DNaseとは異なっていた.

    • 結論:

    • 自身免疫疾患における自己抗体は,DNAを水解する能力を有することがあります.
    • 特定された自己抗体は,核酵素のような活性を持つ免疫グロブリン (IgMとIgG) である.
    • この活動は内生的なDNaseと区別され,ユニークな安定性特性を発揮します.