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Updated: Aug 9, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

2D 13C スピン拡散 NMRによる膜結合後のコリシン ia チャンネルドメインの大規模構造の再編成

Wenbin Luo1, Xiaolan Yao, Mei Hong

  • 1Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.

Journal of the American Chemical Society
|April 28, 2005
PubMed
まとめ

コリシンIaは,膜結合に展開して拡張し,溶けた球状の状態に移行します. この研究は, (13) Cスピン拡散NMRが,膜タンパク質の折りたたみにおける長距離距離変化を明らかにすることを示しています.

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¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
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¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

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NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
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¹³C NMR: ¹H–¹³C Decoupling

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