Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.8K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.8K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

6.2K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
6.2K
Nuclear Export01:42

Nuclear Export

3.7K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.7K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

3.9K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.9K
Rab Proteins01:14

Rab Proteins

4.0K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.0K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

5.9K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
5.9K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Myelin basic protein is an RNA chaperone in microglial nuclear retro-transport.

bioRxiv : the preprint server for biology·2026
Same author

N-Degron-Based PROTAC Targeting PLK1: A Potential Therapeutic Strategy for Cervical Cancer.

Pharmaceutics·2025
Same author

The Replication Function of Rabies Virus P Protein Is Regulated by a Novel Phosphorylation Site in the N-Terminal N Protein-Binding Region.

Viruses·2025
Same author

From transcription to export: mRNA's winding path to the cytoplasm.

Trends in biochemical sciences·2025
Same author

<i>De Novo</i> Designed Cell-Penetrating Peptide Self-Assembly Featuring Distinctive Tertiary Structure.

ACS omega·2024
Same author

To flex or not to flex: oesophageal soft food bolus obstruction in the modern age.

The Journal of laryngology and otology·2024
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
Same journal

Dementia risk in middle-aged people linked to a blood protein.

Nature·2026
Same journal

Daily briefing: What's really happening with trust in science.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: May 3, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.2K

RanGTPによる核輸入複合体解離の構造的基礎

Soo Jae Lee1, Yoshiyuki Matsuura, Sai Man Liu

  • 1MRC Laboratory of Molecular Biology, Hills Rd, Cambridge CB2 2QH, UK.

Nature
|May 3, 2005
PubMed
まとめ
この要約は機械生成です。

核タンパク質の輸入は,インポートリンベータとRanGTPに依存しています. RanGTPがインポートリン-βに結合すると,その構造が変化し,貨物を放出し,核輸送を規制する.

さらに関連する動画

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 26, 2011

20.5K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

3.9K

関連する実験動画

Last Updated: May 3, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.2K
Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 26, 2011

20.5K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

3.9K

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 構造生物学 構造生物学とは

背景:

  • 核タンパク質の輸入は,細胞機能に不可欠である.
  • インポルチン-βは主要な輸送因子として作用し,インポルチン-αをアダプタとして利用する.
  • RanGTPは,核内の輸入複合体の分解を駆動する.

研究 の 目的:

  • 核輸入複合体分解の構造的基礎を解明する.
  • RanGTPがimportin-betaから貨物を解放するメカニズムを理解するために.

主な方法:

  • X線結晶学を用いて,RanGTPと複合した酵母インポートリンベータ (Kap95p) の構造を決定した.
  • 重要な相互作用インターフェースを特定するために,複雑な構造の分析.

主要な成果:

  • RanGTPと複合した全長酵母インポートインベータ (Kap95p) の構造が決定されました.
  • RanGTPスイッチIループとKap95p.のカーボキシ-ターミナルアーチの間に重要な相互作用部位が特定されました.
  • この相互作用は,Kap95pの形状変化を誘導し,貨物の結合を防ぐ.

結論:

  • RanGTPがインポートリン-βに結合すると,アロステル構造の変化が起こります.
  • このメカニズムは,RanGTPが核輸入の際に貨物の放出をどのように促進するかを説明します.
  • この発見は,原子力輸送規制を理解するための構造的基盤を提供します.