関連する実験動画
Updated: Jul 10, 2026

07:44
High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
新しい転写因子により,RNAポリメラーゼII CTDとTFIIDの間の機能的リンクが明らかになりました
A J Koleske1, S Buratowski, M Nonet
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142.
Cell
|May 29, 1992
まとめ
SRB2タンパク質は,遺伝子発現に不可欠な新しい転写因子です. この研究では,RNAポリメラーゼIIのCTDとTFIIDの間の機能的リンクが明らかになり,CTDがRNAポリメラーゼIIを勧誘することを意味する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子発現の規制について
背景:
- RNAポリメラーゼIIのカルボキシ末端領域 (CTD) は転写開始に不可欠ですが,その正確な機能は不明です.
- SRB2はCTD切断変異の抑制剤として特定され,共通の機能的役割を示唆しました.
研究 の 目的:
- RNAポリメラーゼIICTDに関連したSRB2遺伝子の機能を明らかにする.
- SRB2が転写開始に影響を与えるメカニズムを調査する.
主な方法:
- SRB2サプレッサー変異とCTD断絶変異を用いた遺伝子解析.
- 細胞成長と遺伝子発現の欠陥のフェノタイプ分析.
- インビトロ転写アッセイとTFIID結合実験.
主要な成果:
- SRB2とCTDは,機能的な役割を共有しており,それらが存在しない場合でも,同様の成長と遺伝子発現の欠陥によって証明されています.
- SRB2は,転写開始複合体の確立に不可欠な新しい転写因子として作用します.
- SRB2は,トランスクリプション開始複合体と物理的に結合し,TFIIDに特異的に結合します.
結論:
- RNAポリメラーゼIICTDとTATA結合因子TFIIDとの間に機能的なリンクが存在する.
- CTDは,SRB2.2経由で転写開始複合体へのRNAポリメラーゼIIの徴募に関与しています.
関連する概念動画
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
Transcription Initiation
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

