酸化窒素は,フロー条件下での血小板粘着の阻害剤として機能します

J C de Graaf¹, J D Banga, S Moncada

¹Department of Hematology, University Hospital Utrecht, The Netherlands.

Circulation

|June 1, 1992

まとめ

酸化窒素 (NO) は,フロー条件下で,内皮細胞への血小板の粘着を阻害する. この発見は,血小板と血管壁の相互作用を調節する NO の役割を強調しています.

科学分野:

血管生物学血管生物学とは
ヘモスタシスと血栓形成

背景:

酸化窒素 (NO) は,内皮由来リラックス因子 (EDRF) とも呼ばれ,血小板の集約を抑制します.
以前の研究では,NOが静的条件下での血小板粘着を阻害する可能性があることが示唆されました.

研究の目的:

NOが全血中における血小板粘着を,フロー条件下で調節するかどうかを調査する.
エンドセリウム由来および外因的なNOの両方の役割を決定する.

主な方法:

内皮細胞と細胞外マトリックスを含む perfusion システムを用いた.
L-N-モノメチルアルギニン (L-NMMA) とN-イミノエチル-L-オルニチン (L-NIO) を使用した抑制されたNO生産.
異質なNOは,別個の perfusion システム内の多孔膜を通って導入されます.

主要な成果:

NO生成の阻害剤は,濃度に依存した方法で血小板粘着を増加させた.
血小板の粘着は,内皮細胞が存在する場合にのみ増加した.
外因的なNOは,すべての試験された切断率と輸血時間における血小板粘着を抑制した.

結論:

NOは,フロー条件下での血小板粘着の強力な阻害剤です.
NOは,内皮細胞による血小板と血管壁の相互作用の調節に寄与する.

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