細胞内プロアテロゲン現象と細胞外チオール/ディスルファイドによる細胞内再酸化状態によって調節される細胞粘着
1Department of Medicine, Divisions of Cardiology and Pulmonary, Emory University, Atlanta, Ga 30322, USA.
Circulation
|June 2, 2005
まとめ
酸化ストレスが動脈硬化に寄与する. この研究では,血中のシステイン/システィン (Cys/CySS) レドックスポテンシャルが,早期動脈硬化症の発症を調節し,血管疾患リスクマーカーである可能性を示唆しています.
科学分野:
- バイオケミストリー バイオケミストリー
- 細胞生物学 細胞生物学
- 血管生物学 血管生物学
背景:
- 酸化ストレス (Oxidative stress) は,動脈硬化症の発症の要因として知られている.
- 生物学的チオール,例えばシステイン (Cys) とその二硫化システィン (CySS) は,ストレス下で酸化される.
- 早期動脈硬化症における細胞外Cys/CySSリドックスポテンシャルの役割は不明である.
研究 の 目的:
- 早期動脈硬化症の調節における細胞外システイン/シスタイン (Cys/CySS) レドックスポテンシャル (E(h)) の機能を調査する.
- Cys/CySSの酸化が動脈硬化症の発達に因果的に寄与するかどうかを判断する.
主な方法:
- Cys,CySS,グルタチオン (GSH),およびGSSGのレベルを分析して,ヒトの血におけるE (h) を計算した.
- 培養された内皮細胞と単細胞を,細胞外E (h) レベルに変化させました.
- 評価されたH2O2と酸化窒素の生成,核因子-kappaBの活性化,粘着分子発現.
主要な成果:
- 酸化されたCys/CySS E(h) は過酸化水素 (H2O2) の生成を刺激し,核因子-kappaBを活性化し,粘着分子発現を増加させた.
- 酸化されたE (h) は,内皮細胞への単細胞結合を促進した.
- 細胞外E (h) は,細胞表面のチオール/ジスルファイドのリドックス状態とH2O2生成に直接影響した.
結論:
- 細胞外Cys/CySSの酸化還元能力は,早期動脈硬化症の調節に極めて重要です.
- Cys/CySS E (h) は,血管疾患リスクの潜在的なバイオマーカーとして機能する可能性があります.
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