SUMOで修正されたPCNAはSRS2を募集し,S段階の再結合を防止します

Boris Pfander¹, George-Lucian Moldovan, Meik Sacher

¹Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.

Nature

|June 3, 2005

まとめ

SUMO-改変PCNAはSrs2ヘリカーゼと協力して,複製中に望ましくないDNA再結合を防止します. この相互作用は,S相におけるゲノム安定性の維持に極めて重要です.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
細胞生物学細胞生物学

背景:

DNAの損傷は,修復されない場合,複製の停滞とゲノム不安定を引き起こす可能性があります.
増殖細胞核抗原 (PCNA) は,DNA損傷の複製バイパスを可能にするために,ユビキチンによって修正されます.
SフェーズにおけるPCNAの小型ユビキチン関連変容体 (SUMO) 変異の役割は完全に理解されていません.

研究の目的:

SUMOの改変がPCNAに及ぼす機能的影響を調査する.
SUMO改変PCNAとSrs2ヘリケーズとの相互作用を決定するために.

主な方法:

イーストの遺伝子分析. イーストの遺伝子分析.
タンパク質の相互作用を研究するための生化学分析.

主要な成果:

SUMO-改変PCNAはSrs2ヘリカーゼと機能的に協力しています.
Srs2は,特定の結合部位を通って,SUMO修正PCNAと直接相互作用する.
望ましくない再結合を防ぐために,PCNAのSUMO改変はSrs2を勧誘する.

結論:

SUMO改変PCNAは,DNA複製中の再結合を防止する役割を果たします.
SUMO-PCNAとSrs2の相互作用は,ゲノムの安定性を維持するために重要です.

さらに関連する動画

11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

07:11

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Published on: April 16, 2021

関連する概念動画

02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.