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関連する概念動画

DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

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関連する実験動画

Updated: Jul 11, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

ラムダ・インテグラゼによるDNA再結合のアロステリック制御のための構造的基礎.

Tapan Biswas1, Hideki Aihara, Marta Radman-Livaja

  • 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Nature
|June 24, 2005
PubMed
まとめ

細胞機能に不可欠なサイト固有のDNA再結合は,細菌のラムダ・インテグラゼ (lambda-int) によって媒介されます. 結晶構造は,DNA基質と規制DNAを持つラムダ-イント複合体が,再結合のステップを決定し,製品形成を促進する方法を明らかにします.

さらに関連する動画

Identification of Functional Protein Regions Through Chimeric Protein Construction
11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

関連する実験動画

Last Updated: Jul 11, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Identification of Functional Protein Regions Through Chimeric Protein Construction
11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

科学分野:

  • 分子生物学は分子生物学である.
  • 構造生物学 構造生物学とは
  • 遺伝学 遺伝学とは

背景:

  • サイト固有のDNA再結合は,ウイルス統合や遺伝子調節などの細胞プロセスにとって極めて重要です.
  • バクテリオファージ・ラムダは,宿主染色体からそのゲノムを統合/切除するために,インテグラーゼ (lambda-int) を使用する.
  • このプロセスには,lambda-int,アクセサリーDNAサイト,および調節タンパク質が含まれています.

研究 の 目的:

  • バクテリオファージ・ラムダのサイト固有のDNA再結合の構造的メカニズムを解明する.
  • lambda-intがDNA基板と規制要素とどのように相互作用するかを理解する.
  • これらの相互作用がどのように再結合経路を調節するかを明らかにする.

主な方法:

  • X線結晶学を用いて,DNAと複合したラムダ-イントの構造を決定した.
  • 構造は,再結合反応経路の様々な中間物質を捕獲した.
  • 分析は,基板と規制DNAを含む高階複合体に焦点を当てた.

主要な成果:

  • 結晶構造は,lambda-intが2つのドメインを介して同時にDNAに結合し,シナプスを促進する方法を明らかにしました.
  • 構造は,秩序あるDNA鎖の割れ方と交換プロセスを示しています.
  • 付属DNAに結合した相互に絡み合ったアミノ端末ドメインが複合体を形作り,再結合製品を好む.

結論:

  • 構造は,ラムダのサイト固有の再結合のメカニズムに関する原子レベルの洞察を提供します.
  • ラムダ-イントドメインによる同時DNA結合は,シナプスと反応進行の鍵です.
  • lambda-intによる付属DNA結合は,製品形成に向けた反応均衡に影響する.