タイプIIIエフェクタによって誘発されたR遺伝子発現は,米の病気に対する耐性を引き起こす
Keyu Gu1, Bing Yang, Dongsheng Tian
1Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604, Singapore.
Nature
|June 24, 2005
まとめ
植物疾患に対する耐性 (R) 遺伝子と病原体に対する耐性 (avr) 遺伝子が特異性を決定する. この研究では,米のXa27遺伝子発現は,細菌のAvrXa27エフェクターによって誘発され,耐性を授与することが明らかになりました.
科学分野:
- 植物病理学 植物病理学
- 分子遺伝学 分子遺伝学
- 植物と微生物の相互作用
背景:
- 植物疾患に対する耐性は,病原体avr遺伝子を認識するR遺伝子に依存しています.
- 遺伝子対遺伝子の特異性の分子基礎は,まだ完全に理解されていません.
- この相互作用を理解することは,作物保護にとって極めて重要です.
研究 の 目的:
- 米R遺伝子Xa27とその同胞細菌avrXa27遺伝子をクローンし,特徴づけること.
- Xa27媒介性疾患耐性の根底にあるメカニズムを解明する.
- R遺伝子特異性における差異性遺伝子発現の役割を調査する.
主な方法:
- Xa27およびavrXa27遺伝子のクローニング.
- 抵抗性および感受性Xa27アレルの比較分析.
- バクテリアチャレンジアッセイは,米の植物を用いて行われます.
- Xa27遺伝子発現パターンの分析.
- Xa27.27.の胎外発現に関する研究
主要な成果:
- 同様のタンパク質は,抵抗性および感受性Xa27アレルによってコードされます.
- Xa27発現は,Xanthomonas oryzae pv.v.によって誘発された米に特異的に誘発されます. avrXa27.27.を媒介するオリザエ.
- AvrXa27は,核局所化されたタイプIIIエフェクタです.
- 誘発されたXa27の発現は,感染した組織に局在する.
- エクトピックXa27発現は,互換性のある病原菌株に対する耐性を授与する.
結論:
- 米のXa27特異性は,AvrXa27エフェクタによって誘発される異なる遺伝子発現を伴う.
- AvrXa27エフェクターは,Xa27耐性遺伝子の局所発現を誘導する.
- この研究は,植物R遺伝子機能と特異性の分子機構の洞察を提供します.
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