保存されたタンパク質DCN-1/Dcn1pは,C. elegansとS. cerevisiaeにおけるクリン・ネディレーションに必要なものです
Thimo Kurz1, Nurhan Ozlü, Fabian Rudolf
1Institute of Biochemistry, ETH Hönggerberg, 8093 Zürich, Switzerland.
Nature
|July 1, 2005
まとめ
研究者らは,E3ユビキチンリガゼ活性を調節するプロセスであるネディレーションに不可欠なタンパク質であるDCN-1/Dcn1pを特定しました. この発見は,C. elegansと酵母菌におけるタンパク質分解経路の理解を深める.
科学分野:
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
- 細胞生物学 細胞生物学
背景:
- SCF型E3ユビキチンリガゼは,26Sプロテアソームによるタンパク質分解に不可欠です.
- カリン複合体は,Rbx1と共に,触媒核を形成し,基板モジュールを募集する.
- Nedd8の改変 (ネディレーション) と除去 (デネディレーション) は,E3リガゼの活性を調節する.
研究 の 目的:
- クリン・ネディレーションに必要な保存されたタンパク質を特定し,分析する.
- ネディレーション経路におけるDCN-1/Dcn1pの役割を明らかにする.
主な方法:
- C. elegans と S. cerevisiae のタンパク質の特定と分析
- Nedd8結合とクリン関連性を評価するための生化学的測定法.
- ネドジレーションとデンドジレーションの運動性を評価するためのインビボおよびインビトロ実験.
主要な成果:
- UBA類似ドメインとDUF298ドメインを持つ保存されたDCN-1/Dcn1pタンパク質を特定しました.
- DCN-1/Dcn1pはNedd8と直接結合し,カリンと関連している.
- Dcn1pの過剰発現は,ネドジレーションされたクリンレベルを上昇させ,デネドジレーションに影響を与えることなくネドジレーション運動を加速させます.
結論:
- DCN-1/Dcn1pは,クリン・ネディレーションに必要な重要な成分である.
- このタンパク質は,ネディレーションを促進することによって,E3ユビキチンリガゼの活性を正の調節として作用します.
- 発見は,タンパク質のユビキチン化と分解の調節に関する洞察を提供します.
さらに関連する動画
10:50Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Published on: March 14, 2019
08:56In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
Published on: May 5, 2020
関連する概念動画
Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Cohesins
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Condensins
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
Separation of Sister Chromatids
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Catenins
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
