チャネルタンパク質から派生した光作用ナノバルブ
Armagan Koçer1, Martin Walko, Wim Meijberg
1BiOMaDe Technology Foundation, Nijenborgh 4, 9747 AG Groningen, Netherlands.
Science (New York, N.Y.)
|July 30, 2005
まとめ
研究者は,改変した細菌のタンパク質を使用して,光制御分子バルブを開発しました. このナノスケールの装置は,UV光で逆転的に開き,可視光で閉めることができ,分子輸送を正確に制御できます.
科学分野:
- バイオフィジックス 生物物理学
- ナノテクノロジー ナノテクノロジー
- マテリアルサイエンス 材料科学
背景:
- ナノスケールデバイスでは,分子輸送の制御が不可欠です.
- 既存の方法は,しばしば正確な外部制御が欠けている.
- タンパク質ベースのチャネルは,分子レベルで操作する可能性を秘めています.
研究 の 目的:
- ナノスケールのアプリケーションのための光反応性分子バルブを設計する.
- タンパク質チャネルを通じて輸送を外部から光化学的に制御する.
- このバルブをリポソームシステムに組み込み,実用的に使用する.
主な方法:
- 大導電性 (MscL) の機械感受性チャネルをEscherichia coliから光反応性合成化合物で改変する.
- 改変されたMscLをリポソーム膜に埋め込む.
- 長波長紫外線 (366 nm) と可視光を用いて,反転式バルブアクチュエーションを行う.
- 3ナノメートルの孔を通る輸送を監視する.
主要な成果:
- 特定の光の波長を使用した MscL 孔の可逆的な開閉が実証されています.
- エンジニアリングバルブを通して分子輸送に対する外部の光化学制御を達成しました.
- システムのリポソーム封じ込めとの互換性が確認されました.
結論:
- 改良されたMscLタンパク質に基づく機能的な光ゲート分子バルブが成功裏に作成されました.
- このシステムは,ナノスケールでの輸送の正確な制御のための新しいアプローチを提供します.
- 開発された技術は,薬物投与,センシング,ナノデバイスエンジニアリングにおける潜在的な応用があります.
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Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
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