霊長類C型ウイルスの核酸配列は,正常なヒトの組織と新生体のヒト組織に含まれています
1Department of Pathology, University of Chicago, Illinois 60637, USA.
Nature
|May 12, 1977
まとめ
人間の胚性肺線維芽細胞は,霊長類のウイルスに免疫学的に関連しているC型ウイルス,HEL-12を放出します. 証拠によると,このウイルスは汚染物質ではないが,ヒトの癌と関連している可能性があるという.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 腫瘍学 腫瘍学
- 免疫学 免疫学とは
背景:
- 人間の細胞から分離されたC型ウイルスは,霊長類のC型ウイルスとの類似性により,論争を巻き起こしています.
- ヒト細胞におけるC型ウイルスの以前の発見は,繰り返し隔離されたにもかかわらず,しばしば汚染として却下された.
研究 の 目的:
- ヒト胚性肺線維芽細胞から分離されたHEL-12型Cウイルスが偶然の汚染物質ではないことを実証する.
- HEL-12ウイルスと,白血病や全身性狼 eritematosusを含むヒト疾患との潜在的な関連を調査する.
主な方法:
- HEL-12ウイルスの抗原とヒト組織との間の免疫学的クロス反応性アッセイ.
- 骨髄性白血病患者の逆転写酵素に対する特定の阻害性免疫グロブリン検出.
- システミック・ルプス・エリテマトーサス患者におけるグルメラー免疫複合体のHEL-12関連抗原の分析.
- HEL-12細胞におけるプロウイルスのDNA配列と,がん患者のDNAにおける同類のRNA配列を検出するための分子ハイブリダイゼーション技術.
主要な成果:
- 骨髄性白血病患者の循環中の免疫グロブリンが,HEL-12と霊長類C型ウイルス逆転写酵素を阻害した.
- システミック・ルプス・エリテマトーサス患者からのグルメラー免疫複合体には,HEL-12に関連する抗原が含まれていた.
- 検出可能な抗原発現またはウイルスの放出の前に,HEL-12細胞でプロバイラルDNA配列が検出されました.
- HEL-12ウイルスのRNAに同質な核酸配列は,特定の癌患者のDNAで発見されました.
結論:
- HEL-12ウイルスはヒト内生レトロウイルスであり,汚染物質ではない可能性が高い.
- HEL-12に関連する抗原と核酸配列がヒトの組織や癌に存在することは,ヒトの病気における潜在的な役割を示唆しています.
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