シアノバクテリアのミオグルビン
M Potts1, S V Angeloni, R E Ebel
1Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg Va 24061.
Science (New York, N.Y.)
|June 19, 1992
まとめ
ノストック (Nostoc) 町で新型のミオグロビン,シアノグロビンが発見され,窒素を固定するシアノバクテリアである. このヘモプロテインは,真核ミオグロビンと関連しており,窒素不足のマイクロエアロビック条件下で現れます.
科学分野:
- バイオケミストリー バイオケミストリー
- 微生物学 微生物学とは
- 分子生物学は分子生物学である.
背景:
- ミオグロービンは通常,動物の筋肉組織に存在し,酸素の輸送と貯蔵に役立っています.
- プロカリオット,特にサイアノバクテリアにおけるミオグロビン型タンパク質の存在と機能は,まだ十分に理解されていない.
- シアノバクテリアの窒素固定は,酸素レベルに敏感な複雑なプロセスです.
研究 の 目的:
- 窒素を固定するサイアノバクテリアのミオグロビンのようなタンパク質を特定し,特徴づけること ノストック社.
- この新種のシアノバクテリアの血液タンパク質の遺伝的基礎と発現パターンを調査するために.
- 窒素固定中の酸素ホメオスタシスにおけるシアノグロービンの潜在的な役割を調査する.
主な方法:
- ノストック共同体の血液タンパク質の分離と浄化.
- 分子重量決定 (12.5 kDa) を含む生化学的特徴づけ.
- アミノ酸の配列と,既知のミオグロビンとの比較.
- 遺伝子識別 (glbN) と,窒素固定遺伝子 (nifU, nifH) に関するゲノム定位.
- 特定の環境条件 (窒素飢餓,マイクロエアロビックインキュベーション) の下でシアノグロビン発現の検出.
主要な成果:
- 溶解性ヘモプロテイン,シアノグロビンと呼ばれるものは,ノストック市で特定されました.
- シアノグロビンは,パラメシウムやテトラヒメナのような低層のエウカリオットのミオグロビンとシーケンスホモロジーを表しています.
- シアノグロビン遺伝子 (glbN) は,必須の窒素固定遺伝子に隣接しています.
- シアノグロビンは,窒素不足およびマイクロエアロビック条件下にある細胞で特異的に検出されます.
結論:
- ノストック・コミューンは,真核ミオグロビンとの進化的つながりを持つユニークなシアノグロビンを保有しています.
- glbN遺伝子が nif遺伝子の近くにあることが,窒素固定における協調的な役割を示唆しています.
- シアノグロビンの発現は,窒素の利用可能性と酸素濃度によって調節され,窒素酶活性を保護するのに役立つ可能性があります.
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