関連する実験動画

Updated: Jul 26, 2026

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

ビール結合型プロテオリポソーム: 膜タンパク質-リガンド相互作用の nmr 分析のための再構成方法

Mariko Yokogawa1, Koh Takeuchi, Ichio Shimada

  • 1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Journal of the American Chemical Society
|August 25, 2005
PubMed
まとめ

この研究は,親和性ビーズ上の脂質二重層を使用して膜タンパク質の相互作用を研究するための新しい方法を導入し,NMR分析のためのネイティブタンパク質の構造と機能を保存します. この技術は,KcsAカリウムチャネルとアジトキシン2.2の相互作用を成功裏にマッピングしました.

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関連する概念動画

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.