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Updated: Jul 24, 2026

In vivo 19F MRI for Cell Tracking
10:05

In vivo 19F MRI for Cell Tracking

Published on: November 25, 2013

19F NMRベースのスクリーニング実験における感度改善:理論的考察と実験的応用

Claudio Dalvit1, Nicola Mongelli, Gianluca Papeo

  • 1Chemistry Department, Nerviano Medical Sciences, 20014 Nerviano, Milano, Italy. claudio.dalvit@nervianoms.com

Journal of the American Chemical Society
|September 22, 2005
PubMed
まとめ

新しい冷凍フッ素-19探査機は,薬剤発見のためのNMRベースのスクリーニング (FAXSと3-FABS) を強化しています. これにより,感度が向上し,より高いスループットと,サンプル需要が減少したより弱く,より遅い結合物質の検出が可能になります.

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Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
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NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
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Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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Other Nuclides: 31P, 19F, 15N NMR

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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
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Double Resonance Techniques: Overview

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