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16:24
Analyzing and Building Nucleic Acid Structures with 3DNA
Published on: April 26, 2013
ヒトのタンパク質とタンパク質の相互作用ネットワークのプロテオームスケールマップへ
Jean-François Rual1, Kavitha Venkatesan, Tong Hao
1Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA.
Nature
|September 29, 2005
まとめ
研究者は,高通量酵母2ハイブリッドシステムを使用して,ヒトのタンパク質-タンパク質の相互作用をマッピングしました. この初期インタラクトームマップは,病気のタンパク質との新しいつながりを明らかにし,ヒトの生物学と病気のシステムレベルの理解を進めています.
科学分野:
- プロテオミクス プロテオミクスは,プロテオミクスの
- システム生物学 システム生物学
- ヒューマン・インタラクトーム・マッピング
背景:
- タンパク質とタンパク質の相互作用の体系的なマッピング (インタラクトームマッピング) は,モデル生物の理解を深めた.
- インタラクトームマップは,生物学的性質に関連するネットワークの特徴を明らかにします.
- 人間のインタラクトームの地図は,発達と疾患のメカニズムを理解するために不可欠です.
研究 の 目的:
- ヒトのバイナリタンパク質-タンパク質相互作用の初期プロテオームスケールマップを作成する.
- 病気に関連したタンパク質との新しい相互作用と接続を特定する.
- 総合的なヒューマン・インタラクトーム・プロジェクトに寄与する.
主な方法:
- 厳格な,高通量酵母2ハイブリッドシステムを利用しました.
- 約8,100個の開いた読書フレームの製品間の対対の相互作用をテストしました.
- 独立したコアフィニティ浄化アッセイを用いて,検証された相互作用.
主要な成果:
- 約2,800のヒトのバイナリタンパク質-タンパク質相互作用が検出され,CCSB-HI1データセットを形成しました.
- 検出された相互作用の約78%の検証率を達成しました.
- 100以上の疾患に関連したタンパク質への300以上の新しい接続が特定され,利用可能なバイナリ相互作用が70%増加しました.
結論:
- CCSB-HI1データセットは,包括的なヒトインタラクトームマップに向けた重要な一歩を表しています.
- 発見は,システムレベルで人間の発達と病気のメカニズムについての洞察を提供します.
- このインタラクトームマップは,他の生物学的属性と相関し,その有用性を検証します.
関連する概念動画
02:15Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
02:04Protein-protein Interfaces
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
02:04Protein-Protein Interfaces
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
01:24Protein Organization
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
The primary structure of a protein is its amino acid sequence.