ハーピン,植物病原体Erwinia amylovoraによって引き起こされる過敏反応の誘発者
Z M Wei1, R J Laby, C H Zumoff
1Department of Plant Pathology, Cornell University, Ithaca, NY 14853.
まとめ
科学者たちは,過敏反応のような植物防御反応を誘発するErwinia amylovoraのタンパク質であるharpinを分離しました. ハープイン ハープイン ハープイン
科学分野:
- 植物病理学 植物病理学
- 微生物遺伝学とは
- 分子生物学は分子生物学である.
背景:
- Erwinia amylovoraは,バラ色の植物に炎症を引き起こす.
- 過敏反応は植物の防御メカニズムである.
- この反応を誘発するタンパク質は以前は特徴づけられていなかった.
研究 の 目的:
- Erwinia amylovoraからの過敏反応を引き起こすタンパク質を隔離し,特徴づけること.
- このエリシータをコードする遺伝子を特定し,分析する.
- このエリシターの病原性および植物防御における役割を調査する.
主な方法:
- タンパク質エリシータの分離と浄化.
- 生化学的特徴 (pH,熱安定性,分子量).
- 遺伝子クローニング,シーケンシング,およびミュータゲネシス (Tn5tac1).
- パターンの病原性測定法.
- タバコに対する過敏反応の測定.
主要な成果:
- ハーピンと呼ばれるタンパク質のエリシータが分離されました.
- ハーピンは酸性であり,熱に安定し,44 kDaであり,細胞包膜と関連しています.
- ハーピンは葉の葉膜の崩壊を誘発し,タバコの細胞の外部pHを上昇させた.
- ハーピン遺伝子 (hrpN) は,hrp遺伝子クラスター内で特定されました.
- hrpN変異体は非病原性であり,過敏反応を誘発せず,ハーピン生成が欠けていた.
結論:
- ハーピンは,Erwinia amylovora.の主要な毒性因子である.
- ハーピンは,植物における過敏反応を直接誘発する.
- hrpN遺伝子は,ハーピン産生,病原性,過敏反応誘発に不可欠である.
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