関連する実験動画
Updated: Jul 14, 2026
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Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells
Published on: January 12, 2015
クローンされたCdx2欠乏性ブラストキスタから核移転による多能性ES細胞の生成
Alexander Meissner1, Rudolf Jaenisch
1Whitehead Institute and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA.
Nature
|October 18, 2005
まとめ
変異核移転 (ANT) は,不正常な芽細胞を作り,インプラントができないが,カスタマイズされた胚性幹細胞 (ESC) を生成することができる. この研究は,潜在的な治療のための患者特有のESCを生成するためのANTを検証しています.
科学分野:
- 生殖生物学 生殖生物学
- 幹細胞生物学 幹細胞生物学とは
- 発達生物学 発達生物学とは
背景:
- 核移転による胚性幹細胞 (ESC) 誘導は治療的可能性を秘めているが,ブラストシストの破壊を必要とする.
- パーソナライズされた核移転由来ES細胞 (NT-ESCs) は,最近の人間の可行性研究により,免疫不全の治療に有望であることが示されています.
- 変異核移転 (ANT) は,ESC誘導のための非埋め込み性ブラストシストを生成するための提案された方法です.
研究 の 目的:
- カスタマイズされた胚性幹細胞 (ESC) を生成するための改変核移転 (ANT) の概念を実験的に検証する.
- 植え付けができない遺伝子組み換えブラストシストが,依然として多能幹細胞を生成できるかどうかを評価する.
主な方法:
- 核転移は,Cdx2-ターゲティングの短いヘアピンRNA (shRNA) を含むドナー線維芽細胞からマウスブラストシストを作成するために使用されました.
- 生成されたクローンブラストシストの形態学的およびインプラントの評価が行われました.
- 多能の胚性幹細胞は,培養で解剖されたブラストシストから派生した.
主要な成果:
- 生成されたクローン化されたブラストシストは,異常な形状を呈し,機能的なトロフォブラストが欠けていた.
- これらの異常なブラストシストは子宮に埋め込まれることができず,その非生命性の性質が確認されました.
- 発達上の欠陥にもかかわらず,ブラストシストは,実験室で多能性の胚性幹細胞を効率的に生成しました.
結論:
- 変化した核移転 (ANT) は,非埋め込み可能なブラストシストを効果的に生成します.
- このANTアプローチは,生存可能な胚破壊の倫理的な懸念なしに,カスタマイズされた胚性幹細胞 (ESC) を成功裏に生成します.
- ANTは,再生医療の応用のための患者特有のESCを生成するための実行可能な戦略を表しています.
関連する概念動画
00:58Embryonic Stem Cells
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02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
01:14Maintenance of the ES Cell State
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
01:24Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.