Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Lipid-Lowering Drugs: Statins and Miscellaneous Agents01:20

Lipid-Lowering Drugs: Statins and Miscellaneous Agents

Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...
Lipids: Dietary Sources and Requirements01:18

Lipids: Dietary Sources and Requirements

Lipids are an essential component of a balanced human diet. Triglycerides, which make up the majority of dietary lipids, are found in both saturated fats—commonly present in meat, dairy products, and certain tropical plants like coconut, and hydrogenated oils such as margarine and baking shortenings (trans fats)—and unsaturated fats, which are abundant in seeds, nuts, olive oil, and most vegetable oils. The main sources of cholesterol include egg yolks, various meats and organ meats, shellfish,...
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Blood Studies for Cardiovascular System III: Serum Lipid Profile01:25

Blood Studies for Cardiovascular System III: Serum Lipid Profile

Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Atherosclerosis I: Introduction01:30

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
Atherosclerosis III: Management01:26

Atherosclerosis III: Management

Management of atherosclerosis involves an integrated strategy encompassing pharmacological treatment, surgical interventions, lifestyle changes, and nutrition therapy to address the multifactorial nature of the disease.Pharmacological TherapyA cornerstone of atherosclerosis management is the use of pharmacological agents. Statins, such as atorvastatin, are pivotal in inhibiting HMG-CoA reductase, an enzyme that catalyzes an initial step in cholesterol synthesis in the liver. This reduction in...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Calcium Channel Blockade Versus β-Blockade for Hypertension in Heart Failure With Preserved Ejection Fraction: A Randomized Crossover Trial.

Hypertension (Dallas, Tex. : 1979)·2026
Same author

RACGAP1 as a Circulating Biomarker of Atrial Fibrillation in Heart Failure: A Dual-Cohort Proteomic Study.

Circulation. Arrhythmia and electrophysiology·2026
Same author

Longitudinal dynamics of organ-specific proteomic aging clocks over a decade of midlife.

bioRxiv : the preprint server for biology·2026
Same author

Arterial Stiffness and Renal Outcomes: Insights into the Vascular-Renal Connection.

Kidney360·2026
Same author

Proteomic Risk Score for Prediction of Incident Hypertension.

Hypertension (Dallas, Tex. : 1979)·2026
Same author

Dephospho-Uncarboxylated Matrix Gla-Protein Is Associated With Adverse Outcomes in Heart Failure.

Circulation. Heart failure·2026

関連する実験動画

Updated: Jun 22, 2026

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

血清の細胞アシルCoA:コレステロールアシルトランスファーゼ活性を下げる能力は,心血管疾患のアウトカムを予測する.

Julio A Chirinos1, Juan P Zambrano, Simon Chakko

  • 1Miller School of Medicine, University of Miami, Miami, FL 33138, USA.

Circulation
|October 19, 2005
PubMed
まとめ

血清コレステロール流出活性 (コレステロールをエステル化のために低下させる能力によって測定される) は,独立して主要な有害心血管疾患 (MACE) と男性における死亡を予測します. この発見は,心血管リスク評価に関する新しい洞察を提供します.

さらに関連する動画

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

関連する実験動画

Last Updated: Jun 22, 2026

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

科学分野:

  • 心血管医学 心血管医学
  • バイオケミストリー バイオケミストリー
  • 脂質代謝 脂質代謝とは

背景:

  • 血清コレステロール流出活動と冠動脈疾患 (CAD) の関連性を調査した.
  • コレステロール流出と主要な心血管疾患 (MACE) の有害事象および死亡のリスクとの関連を調べた.

研究 の 目的:

  • 血清コレステロール流出活動が血管学的CADの存在を予測するかどうかを判断する.
  • コレステロール流出活動とMACEおよび死亡のリスクとの関連性を評価する.

主な方法:

  • 冠動脈血管撮影を受けた168人の男性を研究した.
  • 患者の血清とヒトの皮膚線維芽細胞を用いて,実験室内でのコレステロール流出活性を測定した.
  • 血清のコレステロールを減少させる能力によって定義されたエフフルス活性,アシルCoA:コレステロールアシルトランスファーゼ (ACAT) エステル化のために利用可能なコレステロール.
  • MACEと死亡を追跡するために,4.5年間患者を追跡しました.

主要な成果:

  • ACAT活性における血清誘発の変化は,HDLレベルやCADの存在と相関していなかった.
  • ACAT活性変化の最高端の患者には,MACE (HR, 2.15) と死亡 (HR, 2.23) のリスクが著しく高かった.
  • これらの相関は,LDL,HDL,C反応性タンパク質などの他のリスクマーカーとは無関係でした.

結論:

  • ACATのエステル化のために細胞のコレステロールを枯渇させる血清の能力は,MACEと死亡の強い,独立した予測因子です.
  • この活動にはATP結合カセットトランスポーターA1 (ABCA1) 媒介のエフフルスが含まれていると推測されています.
  • 血清内のACATに影響を与えるHDL粒子の増加は,生体内でのABCA1活性に制限されていると仮定した.