LIN-12/Notchの活性化は,C. elegansにおけるVavのマイクロRNA媒介下調調節につながります
1Integrated Program in Cellular, Molecular, and Biophysical Studies, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, 701 West 168th Street, Room 720, New York, NY 10032, USA.
Science (New York, N.Y.)
|October 22, 2005
まとめ
研究者らは,Caenorhabditis elegans vulval前駆細胞 (VPC) のLIN-12,mir-61,VAV-1を含むフィードバックループを発見した. このループは,LIN-12のシグナル伝達を強化して,発達中の2度目の細胞運命を促進します.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- 細胞と細胞の相互作用と信号経路の交響は,細胞の運命の仕様には極めて重要です.
- Caenorhabditis elegansでは,前駆細胞 (VPC) は,シグナリングレベルに基づいて,異なる運命を (1度,2度) 採用する.
研究 の 目的:
- VPC運命の仕様の新しいレギュレータを特定する.
- 2度VPC仕様におけるフィードバックループの基礎となる分子メカニズムを解明する.
主な方法:
- LIN-12の直接的な転写標的としてのマイクロRNA (mir-61) の識別.
- 2度VPCの運命を促進する mir-61の役割の分析.
- Mir-61の標的であるvav-1の特定と,VAV-1のダウンレギュレーションにおけるその役割.
主要な成果:
- LIN-12は,mir-61.1の発現を直接制御しています.
- mir-61は2度VPCの運命を促進する.
- VAV-1はmir-61の標的であり,そのダウンレギュレーションはLIN-12の活動を強化し,2度の運命を強化します.
結論:
- LIN-12,mir-61,およびVAV-1を含むフィードバックループが特定されています.
- このループは,LIN-12シグナリングを増幅し,2度VPC運命の堅固な仕様を確保します.
関連する概念動画
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...