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関連する概念動画

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Mutations01:39

Mutations

Overview
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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Updated: Jul 8, 2026

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
12:43

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions

Published on: November 12, 2017

ファシキュラリニンによるDNA損傷

Sanjay Dutta1, Hideki Abe, Sakae Aoyagi

  • 1School of Pharmacy, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192-0392, Japan.

Journal of the American Chemical Society
|October 27, 2005
PubMed
まとめ

ファシキュラリン (fasicularin) は,海からの新しいアルカロイドで,グアニンの残基をアルキル化することによってDNAを損傷します. この天然化合物は,特定の化学療法薬と同様のアジリジニウムイオンを形成し,DNAを損傷する天然製品に関する新しい洞察を提供します.

科学分野:

  • 自然製品化学 自然製品化学
  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー

背景:

  • ファシキュラリンは,アシディアン * Nephteis fasicularis * * から分離されたチオシアネートを含む新型アルカロイドです.
  • 以前の研究では,ファシキュラリンはDNA損傷によって潜在的に細胞毒性を持つことを示唆していました.

研究 の 目的:

  • ファシキュラリンがDNA損傷を誘発するメカニズムを解明する.
  • ファシキュラーリン媒介のDNA分裂に関与する化学種を特徴付けるため.

主な方法:

  • ファシキュラーリンで処理された5'-32Pラベル付きDNAデュプレックスのシーケンスゲル解析.
  • DNAの病変と反応性中間物質を特定するための生化学的分析.

主要な成果:

  • ファシキュラリンの治療は,DNA内のグアニン残留で選択的な鎖裂けを引き起こしました.
  • DNA損傷のメカニズムは,ファシキュラリン由来アジリジニウムイオンによるグアニンのアルキル化を含む.
  • このアジリジニウムイオン形成は,臨床的に使用されている抗がんアルキル化剤と類似しています.

結論:

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Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
10:16

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Published on: January 25, 2019

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
05:18

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Published on: June 9, 2020

関連する実験動画

Last Updated: Jul 8, 2026

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
12:43

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions

Published on: November 12, 2017

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
10:16

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Published on: January 25, 2019

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
05:18

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Published on: June 9, 2020

  • ファシキュラリンは,DNAをアルキル化するアジリジニウムイオンを生成する最初の天然製品です.
  • ファシキュラリンの作用機構は,抗がん薬の開発に関連するDNAアルキル化のための新しい天然製品モデルを提供します.