トライパノソーマ・ブルセイにおけるゴルギの複製には,セントリン2が必要です

Cynthia Y He¹, Marc Pypaert, Graham Warren

¹Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.

Science (New York, N.Y.)

|October 29, 2005

まとめ

セントリンは細胞構造に不可欠です. トライパノソーマ・ブルセイでは,TbCentrin2は,新しいゴルギ関連構造に局所化し,新しいゴルギ組立におけるその役割を明らかにします.

科学分野:

細胞生物学細胞生物学
寄生虫学とは,寄生虫学である.
分子生物学は分子生物学である.

背景:

セントリンとは,Trypanosoma bruceiの基礎体複製とフラゲル形成に不可欠な保存されたセントロソームタンパク質です.
T. bruceiのセンターゾームには,運動のために鞭毛を核化する基礎体が含まれています.

研究の目的:

センターソームにおける既知の役割を超えて,TbCentrin2の局所化と機能を調査する.
TbCentrin2がT. brucei.におけるゴルギ器官の複製と組み立てにおける役割を決定する.

主な方法:

TbCentrin2の局所化を視覚化するための免疫光顕微鏡.
RNA干渉 (RNAi) によって,TbCentrin1とTbCentrin2.0が枯渇する.
タンパク質の枯渇後の基礎体とゴルギの重複の分析.

主要な成果:

TbCentrin2は,ゴルギ装置の隣接する独特の双葉構造で特定されました.
TbCentrin2構造の片方の葉は,既存のゴルギと関連しており,もう片方は,形成中のゴルギと関連しています.
TbCentrin1の枯渇は,ベースボディの複製を特異的に抑制しました.
TbCentrin2の枯渇は,基礎体複製とゴルギ複製の両方を抑制しました.

結論:

TbCentrin2は,ゴルギ装置の複製において,これまで認識されていない機能を持っています.
ベースボディから分離したセントリン2を含む構造は,新しいゴルギの組み立て場所として機能します.
これらの発見は,真核細胞の細胞複製とオルガネルの生体生成における,既知のセントリンの役割を拡大している.

さらに関連する動画

09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

11:26

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

関連する概念動画

02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...

01:12

Golgi Matrix Proteins

Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...

02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...

01:26

Transport Across the Golgi

While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...

02:25

Centrosome Duplication

01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...

関連する実験動画