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関連する概念動画

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

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関連する実験動画

Updated: Jul 2, 2026

Measuring Replicative Life Span in the Budding Yeast
12:41

Measuring Replicative Life Span in the Budding Yeast

Published on: June 25, 2009

Sir2は,極度の寿命延長をブロックします.

Paola Fabrizio1, Cristina Gattazzo, Luisa Battistella

  • 1Andrus Gerontology Center and Department of Biological Sciences, University of Southern California, Los Angeles, California 90089, USA.

Cell
|November 16, 2005
PubMed
まとめ

Sir2 (サイレント・インフォメーション・レギュレータ2) の無活性化により,レプリケーション寿命への影響とは対照的に,酵母菌の時間的寿命が延長されます. これにはエタノール代謝とストレス遺伝子のアップレギュレーションが含まれています.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • 老化に関する研究

背景:

  • Sir2 (サイレント・インフォメーション・レギュレータ2) は,種間の寿命とストレス反応に影響を及ぼす保存型デサセチラゼである.
  • 酵母では,Sir2は複製寿命を維持するために不可欠であり,投与量の増加は老化を遅らせます.

研究 の 目的:

  • 酵母における時間的寿命の調節におけるSir2の役割を調査する.
  • Sir2が酵母老化に及ぼす影響の基礎にある分子メカニズムを理解するために.

主な方法:

  • SIR2遺伝子 (sir2Δ) の削除を含む遺伝子操作.
  • カロリー制限やSch9またはRas経路の変異を含む様々な条件下での時間的寿命の分析.
  • エタノールの吸収/分解の測定と遺伝子発現プロファイリング (ストレス抵抗性および胞子化遺伝子).

主要な成果:

  • Sir2機能の喪失は,酵母菌の時間的寿命を大幅に延長し,複製寿命における役割とは正反対の効果である.
  • Sir2の無活性化により,エタノール消費量が増加し,ストレス抵抗性および胞子発生遺伝子のアップレギュレーションが起こります.
  • sir2Δ変異体の寿命を延ばすには,深刻なカロリー制限や追加の遺伝子変異が必要です.

さらに関連する動画

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates
12:23

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates

Published on: March 18, 2011

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

関連する実験動画

Last Updated: Jul 2, 2026

Measuring Replicative Life Span in the Budding Yeast
12:41

Measuring Replicative Life Span in the Budding Yeast

Published on: June 25, 2009

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates
12:23

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates

Published on: March 18, 2011

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

結論:

  • 酵母菌の寿命に対するSIR2の影響は,文脈に依存しており,複製的および時間的老化に相反する効果があります.
  • 老化におけるSir2の役割は複雑で,代謝経路とストレス反応経路の複雑な調節を伴う可能性があります.
  • 発見は,Sir2型脱エチラゼが,より高いエウカリウトの老化過程において,複雑で,潜在的に対極的な役割を果たす可能性があることを示唆している.