Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

17.4K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
17.4K
Destabilization of Microtubules01:45

Destabilization of Microtubules

2.9K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.9K
Microtubule Instability02:17

Microtubule Instability

5.0K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.0K
Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

3.1K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
3.1K
Anaphase A and B01:39

Anaphase A and B

4.3K
Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
4.3K
Disassembly of Intermediate Filaments01:35

Disassembly of Intermediate Filaments

2.0K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.0K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Understanding microtubule dynamics: The synergy of technology, theory, and experiment.

The Journal of cell biology·2025
Same author

Biomimetic Mineralization Strategies to Construct Heparin/Titanium Dioxide Anticoagulant and Antimicrobial Coatings for Blood Contact Devices.

ACS applied bio materials·2025
Same author

Replisome passage through the cohesin ring.

Cell·2025
Same author

Ndc80 complex, a conserved coupler for kinetochore-microtubule motility, is a sliding molecular clutch.

Science advances·2025
Same author

Ndc80 complex, a conserved coupler for kinetochore-microtubule motility, is a sliding molecular clutch.

bioRxiv : the preprint server for biology·2025
Same author

Author Correction: An extrinsic motor directs chromatin loop formation by cohesin.

The EMBO journal·2025
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: May 5, 2026

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 16, 2014

13.9K

マイクロチューブルを分解することによって,力の生成.

Ekaterina L Grishchuk1, Maxim I Molodtsov, Fazly I Ataullakhanov

  • 1MCD Biology Department, University of Colorado at Boulder, Colorado 80309-0347, USA.

Nature
|November 18, 2005
PubMed
まとめ
この要約は機械生成です。

デポリメリゼーションマイクロチューブル (MTs) は,付着したビーズに大きな引力をかけ,かなりの力を発生させます. この微小管のダイナミクスメカニズムは,細胞分裂中の染色体運動の主な原動力かもしれません.

さらに関連する動画

Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
07:47

Directly Measuring Forces Within Reconstituted Active Microtubule Bundles

Published on: May 10, 2022

1.5K
Self-Assembly of Microtubule Tactoids
08:49

Self-Assembly of Microtubule Tactoids

Published on: June 23, 2022

4.6K

関連する実験動画

Last Updated: May 5, 2026

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 16, 2014

13.9K
Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
07:47

Directly Measuring Forces Within Reconstituted Active Microtubule Bundles

Published on: May 10, 2022

1.5K
Self-Assembly of Microtubule Tactoids
08:49

Self-Assembly of Microtubule Tactoids

Published on: June 23, 2022

4.6K

科学分野:

  • 細胞生物学 細胞生物学
  • バイオフィジックス 生物物理学
  • 細胞骨格のダイナミクス

背景:

  • マイクロチューブル (MTs) は,細胞の形状,運動,臓器の運搬に関与する重要なユーカリオット細胞骨格の構成要素である.
  • 運動酵素はMTを結合して動きを促しますが,MTダイナミクス自体も運動性に寄与します.
  • MTが蓄積された化学エネルギーを機動性のための機械的な作業に変換するメカニズムは完全に理解されていません.

研究 の 目的:

  • マイクロチューブルのデポリメリゼーションによって発生する機械的力を調査する.
  • 微小管のダイナミクスが細胞の動きを直接駆動できるかどうかを判断する.
  • 単一のデポリメリジングマイクロチューブルによって生成される力を定量化するために.

主な方法:

  • ビオチン-アビジン結合を用いて,ガラスマイクロビーズをチューブリンポリマーに結合させる.
  • マイクロチューブルのデポリメリゼーション中にレーザーピンチを使用して,ビーズの移動と力を測定する.
  • 微小管で発生する力を分子機械モデルで分析する.

主要な成果:

  • デポリメリゼーションするマイクロチューブルが,結合したマイクロビーズに短い引く力を発揮することが観察されました.
  • 単一のデポリメリジングマイクロチューブルは,モーター酵素の約10倍の力を生み出すことができます.
  • 実験的な結合法により,微小管の分解がわずかに遅れた.

結論:

  • 微小管の脱ポリメリゼーションは力強い力源であり,潜在的に染色体運動を誘導する.
  • このメカニズムは,細胞プロセスにおけるモーター酵素に対する代替的または補完的な力発生器を提供します.
  • 生理学的要因は,微小管のダイナミクスを調節して,細胞の運動性をin vivoで調節する可能性があります.