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Updated: Jul 6, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

カテコール1,2-ダイオキシゲナーゼの無酸素酵素基板複合体のスペクトロスコーピック研究

Geoff P Horsman1, Andrew Jirasek, Frédéric H Vaillancourt

  • 1Department of Biochemistry, The University of British Columbia, Vancouver, Canada.

Journal of the American Chemical Society
|December 1, 2005
PubMed
まとめ

この研究では,カテキールは,エクストラディオール酵素とは異なり,イントラディオール酸化酸化酵素で鉄にダイアニオンとして結合することを明らかにしました. この発見は,スペクトロスコピーとDFTによって裏付けられ,酵素機構と基板結合を明確にします.

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