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Updated: May 11, 2026
12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
APC/CとCBP/p300は,転写と細胞サイクル進行を調節するために協力しています
Andrew S Turnell1, Grant S Stewart, Roger J A Grand
1Cancer Research UK Institute for Cancer Studies, The Medical School, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. A.S.Turnell@bham.ac.uk
Nature
|December 2, 2005
まとめ
アナフェーズ促進複合体/サイクロソーム (APC/C) はCBP/p300共同活性化剤と相互作用し,細胞サイクル進行と転写に影響を与えます. この相互作用は,APC/C機能に不可欠であり,細胞の変容に影響を与えます.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- アナフェーズ促進複合体/サイクロソーム (APC/C) は,ミトーシス経由の細胞サイクル進行を調節する重要なE3ユビキチンリガゼであり,G1.
- 細胞サイクル制御におけるその役割は確立されているが,他のAPC/C機能は理解されていない.
研究 の 目的:
- 細胞周期調節を超えたAPC/C構成要素の新たな機能を調査する.
- APC/Cと共同活性化剤CBP/p300との相互作用とその機能的影響を解明する.
主な方法:
- APC5/APC7およびCBP/p300結合を研究するためのタンパク質相互作用アッセイ.
- CBP/p300アセチルトランスフェラーゼ活性と転写活性を測定するアッセイ.
- APC/C機能におけるCBPの役割を評価するためのRNA媒介干渉.
- E1A媒介による細胞変容の分析.
主要な成果:
- APC/CコンポーネントであるAPC5とAPC7は,保存ドメインを介して,共活性化剤CBPとp300と直接相互作用する.
- この相互作用はCBP/p300アセチルトランスフェラーゼの活性を高め,転写を活性化します.
- APC5とAPC7は,CBP/p300に依存した方法でE1A媒介による変換を抑制する.
- CBPは,APC/Cの活動に不可欠であり,CBPの減少はE3リガゼの活性とミトスの進行を低下させる.
結論:
- APC/CコンポーネントであるAPC5とAPC7は,CBP/p300と機能的な複合体を形成し,転写と細胞変換に影響を与えます.
- これらの発見は,成長調節におけるAPC/Cの新たな役割を明らかにし,APC/Cのコンポーネントが変容中に標的となる可能性があることを示唆しています.
- CBPは,APC/Cユビキチンリガゼ活性と細胞サイクル進行の重要なレギュラーである.
関連する概念動画
01:23Negative Regulator Molecules
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
01:28The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...