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Updated: Jul 14, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

グラファイト電極マイクロアレイの改変のためのダイアゾニウムタンパク質アダクト:直接的および宛先の電気化学的不動化

Benjamin P Corgier1, Christophe A Marquette, Loïc J Blum

  • 1Laboratoire de Génie Enzymatique et Biomoléculaire, UMR 5013 EMB2, CNRS Université Claude Bernard Lyon 1, Bât CPE, 43, boulevard du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France.

Journal of the American Chemical Society
|December 22, 2005
PubMed
まとめ

この研究は,ダイアゾニウムカチオン電解を直接改変されたタンパク質に使用してタンパク質を固定するための新しい方法を導入しています. このテクニックは,敏感な免疫バイオチップアプリケーションのためのスクリーンプリントされた電極上のタンパク質の精密な電気アドレスを可能にします.

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Metal-Ligand Bonds

The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
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Diazonium Group Substitution: –OH and –H

Nitrous acid, a weak acid, is prepared in situ via the reaction of sodium nitrite with a strong acid under cold conditions. This nitrous acid prepared in situ reacts with primary arylamines to form arenediazonium salts. Such reactions are known as diazotization reactions. As shown in Figure 1, the formation of arenediazonium salts begins with the decomposition of nitrous acid in an acidic solution to give nitrosonium ions.
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1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism

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EDTA: Auxiliary Complexing Reagents

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Masking and Demasking Agents

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Complexometric Titration: Ligands

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