ユーカリオットの遺伝子発現における外部的多様性の起源

Dmitri Volfson¹, Jennifer Marciniak, William J Blake

¹Department of Bioengineering, University of California San Diego, La Jolla, California 92093, USA.

Nature

|December 24, 2005

まとめ

細胞の多様性は,遺伝子発現の多様性から生じる. この研究は,細胞の違いを理解するために不可欠な,集団動態と規制ネットワークを含む,外的な変動の2つの主要な源を特定しています.

科学分野:

分子生物学は分子生物学である.
システム生物学システム生物学
遺伝学遺伝学とは

背景:

クローン細胞集団内の変異性遺伝子発現は,進化や病気などの重要な生物学的プロセスに影響を与えます.
外的な要因は,複数の遺伝子に影響を及ぼし,表現の変動に大きく寄与しますが,その起源はまだ十分に研究されていません.

研究の目的:

外在的な遺伝子発現の変動性の生物学的起源を特定する.
遺伝子規制ネットワークが細胞の多様性にどのように寄与するかを調査する.

主な方法:

計算モデルとSaccharomyces cerevisiaeからの光データを使用した.
発現の変動性を定量化するために,複数のプロモーター遺伝子挿入からのデータを分析した.

主要な成果:

外的な変動の2つの主要な源を特定した:遺伝子発現と集団動態の結合と共通の上流転写因子.
規制ネットワークが上流の規制当局からのノイズを増幅することができ,標的遺伝子の外部ノイズに寄与することを実証しました.
集団動態による表現変動の下限を確立した.

結論:

外的な遺伝子発現の変動性は,人口動態と規制ネットワーク構造の両方から生じます.
遺伝子規制ネットワークと集団の異質性の相互作用を理解することは,細胞の多様性を説明する鍵です.

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