関連する実験動画
Updated: Jul 6, 2026
08:33
Ubiquitin Chain Analysis by Parallel Reaction Monitoring
Published on: June 17, 2020
APCによるマルチウビキチネーションのプロセシビティは,基板分解の順番を決定する
Michael Rape1, Sashank K Reddy, Marc W Kirschner
1Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Cell
|January 18, 2006
まとめ
アナフェーズ促進複合体 (APC) は,細胞サイクル中に基板の分解を命じる. サブストラットプロセシビティ,すなわちユビキチン鎖の追加速度が,分解のタイミングを決定し,適切な細胞サイクル進行を保証します.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- アナフェーズ促進複合体 (APC) は,細胞循環の調節,ミトーシスとG1相の調整に不可欠です.
- APCは細胞循環の重要なレギュレータを順次退化させるが,基質の順序付けのメカニズムは不明である.
研究 の 目的:
- APCが基質のユビキチン化と分解の時間配列を確立するメカニズムを解明する.
- APC媒介の細胞サイクル制御における基質プロセシビティの役割を調査する.
主な方法:
- APCによる基質マルチウビキチネーションの相対的なプロセシビリティを in vitroで調査した.
- サブストラットプロセシビティがユビキチン化と分解のタイミングに与える影響を in vivoで評価した.
- Dボックスモチーフが基質の多重ビキチネーションプロセス性に及ぼす影響を分析した.
主要な成果:
- APCによる基質の順序化は,マルチウビキチネーションの相対的な過程性に依存する.
- 単一の結合イベントでユビキチン鎖を取得するプロセシブ基板は,好ましくは,より早くユビキチン化され,分解されます.
- サブストラットDボックスは,マルチウビキチネーションのプロセシビティに大きな影響を及ぼし,固有のメカニズムを示しています.
結論:
- APC媒介の基質の順序化は,マルチウビキチネーションの固有のプロセス性によって支配され,Dボックスのような基質の特徴の影響を受けます.
- このプロセスは,動的校正に類似しており,細胞サイクル調節器の正確な時間的な制御を保証します.
関連する概念動画
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02:58Regulated Protein Degradation
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
01:13The Proteasome
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...