RNA結合タンパク質FCAは,アブシシ酸受容体である
Fawzi A Razem1, Ashraf El-Kereamy, Suzanne R Abrams
1Department of Plant Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
Nature
|January 20, 2006
まとめ
RNA結合因子であるFCAタンパク質は,植物ホルモンアブシシ酸 (ABA) の高親和受容体として作用する. この発見は,ABABAを明らかにしています.
科学分野:
- 植物生物学 植物生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- アブシシ酸 (ABA) は,さまざまな生理学的プロセスを調節する重要な植物ホルモンです.
- ABAシグナル伝達のための正確な分子機構と受容体は,ほとんど解明されていないままです.
- ABA応答遺伝子はRNAの関与を示唆しているが,直接的なABA結合タンパク質は特定されていない.
研究 の 目的:
- アブシシ酸 (ABA) の難解な受容体を特定する.
- ABAの知覚とシグナル伝達におけるRNA結合タンパク質の役割を調査する.
- ABAを花開などの植物発達プロセスと結びつける分子メカニズムを解明する.
主な方法:
- 候補タンパク質へのABA結合をテストするための生化学的測定法.
- 花期突然変異体の遺伝子分析. 花期突然変異体の遺伝子分析.
- RNA結合アッセイとインビトロスプライシングアッセイ.
- 遺伝子発現とRNA処理に対するABA効果の分析.
主要な成果:
- 花開の時間を調節するRNA結合タンパク質であるFCAは,高親和性ABA受容体として特定されました.
- FCAへのABA結合はステレオ特異であり,受容体のような運動性を表している.
- ABA-FCAの相互作用は,FCAによって媒介されるRNA処理に直接影響を及ぼします.
- この相互作用は,自律的な花路における下流のイベントに影響を与え,花開の時間に影響を与えます.
結論:
- FCAはアブシシ酸 (ABA) の直接受容体として機能する.
- FCAによるABAの知覚は,ホルモンシグナル伝達とRNA代謝を統合する.
- このメカニズムは,ABA,RNA処理,および植物の開花時間の調節の間の新しいリンクを提供します.
関連する概念動画
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
tRNA Activation
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
The ADP/ATP Carrier Protein
ADP/ATP carrier or AAC protein is the most abundant carrier protein in the inner mitochondrial membrane. It transports large quantities of ADP and ATP, equivalent to the average human body weight, every day. Among other transporters, ACC protein is one of the best-studied members of the mitochondrial carrier protein family. The ADP/ATP carrier protein comprises two transmembrane helices connected to a loop and a single alpha-helix on the matrix side. It switches between two conformational...
Directing Proteins to the Rough Endoplasmic Reticulum
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
Tail-anchoring of Proteins in the ER Membrane
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
tRNA Activation
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...


