evi5腫瘍遺伝子は,アナフェーズ促進複合阻害剤emi1を安定させることで,サイクリン蓄積を調節する
Adam G Eldridge1, Alexander V Loktev, David V Hansen
1Department of Cancer Biology, Stanford University School of Medicine, CA 94305, USA.
Cell
|January 28, 2006
まとめ
腫瘍遺伝子のEvi5は,アナフェーズ促進複合体/サイクロソーム (APC/C) 阻害体Emi1を安定させ,細胞サイクル進行を早期に防ぐ. Evi5 について
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 腫瘍遺伝子 (オンコゲネス) とは
背景:
- アナフェーズ促進複合体/サイクロソーム (APC/C) は,細胞サイクル進行において極めて重要です.
- Emi1はAPC/Cの主要な阻害体であり,ミトーシスへの入り口を調節する.
- 適切なEmi1レベルを維持することは,細胞サイクルエラーを防ぐために不可欠です.
研究 の 目的:
- EMI1の蓄積のレギュレータを特定する.
- Evi5 が Emi1 の安定性を制御するメカニズムを解明する.
- 細胞周期の忠誠性を維持するEvi5の役割を理解するために.
主な方法:
- 同免疫プレシピテーションにより,Emi1結合タンパク質を特定する.
- タンパク質の分解経路の分析.
- 細胞サイクル分析とセンターソーム複製アッセイ.
主要な成果:
- Evi5は,Emi1.1を安定させるEmi1結合タンパク質として特定されました.
- Evi5はSCF ((betaTrCP) 媒介のEmi1のユビキチン化と分解を阻害する.
- Evi5の消去は,早期のAPC/Cの活性化,細胞サイクル停止,ミトスの破滅につながる.
結論:
- Evi5は,S/G2段階において,Emi1の決定的な安定化因子として作用する.
- Evi5とPoloのようなキナーゼの活動とのバランスは,Emi1とサイクリンレベルを調節します.
- このバランスは,正確な細胞サイクル進行とミトスの忠誠性を確保するために非常に重要です.
関連する概念動画
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
01:12Loss of Tumor Suppressor Gene Functions
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
02:40Cancer Stem Cells and Tumor Maintenance
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
01:12Loss of Tumor Suppressor Gene Functions
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
02:40Cancer Stem Cells and Tumor Maintenance
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...