アクティヴィン受容体の表現クローニング,予測されたトランスメブランセリンキナーゼ
1Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037.
Cell
|June 14, 1991
まとめ
研究者らは,マウスの下垂体細胞からアクティビン受容体cDNAをクローンした. この受容体は高い親和性でアクティビンAと結合し,セリン/スレオニンキナーゼドメインを有しており,細胞信号伝達経路における役割を示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- セルラー・シグナリング
- エンドクリノロジー エンドクリノロジー
背景:
- アクティビンは,発達と下垂体機能を含む多様な生物学的プロセスを調節します.
- アクティビンの作用の細胞メカニズムを理解するには,その受容体の識別が必要です.
研究 の 目的:
- マウスのAtT20細胞からアクティビン受容体をコードするcDNAをクローンして特徴づけること.
- 特定されたアクティビン受容体の結合特性と構造特性を調査する.
主な方法:
- 125I-アクティビンA結合のためのCOS細胞トランスフェクタントのスクリーニング.
- アクティヴィン受容体をコードするcDNAのクローニング.
- アフィニティ・バインディング・アッセイと競争調査.
主要な成果:
- 494アミノ酸アクティビン受容体をコードするcDNAが分離されました.
- この受容体は,細胞外リガンド結合ドメイン,膜外ドメイン,細胞内セリン/スレオニンキナーゼドメインを特徴としています.
- 125I-アクティビンAは高い親和性 (180 pM) で結合し,アクティビンA,アクティビンB,およびインヒビンAと競合していましたが,TGF-β1.1はそうではありませんでした.
結論:
- キナーゼ活性を持つ新しいアクティビン受容体は,マウスの皮質otropic 細胞で特定されました.
- 受容体のキナーゼドメインは,C. elegans daf-1遺伝子の産物と同一性を示し,シグナル伝達経路が保存されていることを示唆しています.
- この発見は,アクティビン媒介細胞調節の分子機構の洞察を提供します.
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