ドロソフィラトウィスト遺伝子の配列特異的トランザクティベーションは,背面遺伝子の産物によって行われます
C Thisse1, F Perrin-Schmitt, C Stoetzel
1Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France.
Cell
|June 28, 1991
まとめ
背面タンパク質は,ドロソフィラ胚のトウィスト遺伝子を活性化させ,背面-腹面軸に沿った発達を制御します. このDNA結合タンパク質は,NF-カッパBに似たモチーフを認識する.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- 母子のドーサル遺伝子は,ドロソフィラ胚のドーサル・ベントラル軸の形成に不可欠なタンパク質をコードする.
- 以前の研究では,ドーサルがジゴティック遺伝子ツイストを活性化することを示唆していたが,直接的な証拠は欠けていた.
研究 の 目的:
- ツイスト遺伝子発現を制御する規制配列を定義する.
- ツイストプロモーターの活性化における背中のタンパク質の役割を確認するために.
- ドーサルのDNA結合特性を特徴付けるため.
主な方法:
- フェノタイプの救助実験.
- ツイスト-ベータ-ガラクトシダース発現パターンの分析.
- トランジエントコトランスフェクションアッセイは,プロモーター活性化を研究するためのものです.
- 背面認識モチーフを特定するためのDNA結合アッセイ.
主要な成果:
- ツイスト表現のための特定の規制配列が特定されました.
- 背中のタンパク質は,トウィストプロモーターを直接活性化することが示されました.
- Dorsalは,配列特異的なDNA結合タンパク質であると確認されました.
- ドーサルのDNA結合モチーフは,哺乳類のNF-kappa B.のモチーフに似ています.
結論:
- ドルサルは配列特異的な転写因子として作用し,twist遺伝子を活性化します.
- このメカニズムは,ドロソフィラ胚の背中中軸のパターンの形成に不可欠です.
- この発見は,ドロソフィラと哺乳類のシステムを結びつける,転写調節における進化的保存を明らかにしています.
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