牛のパピローマウイルスE5のオンコタンパク質は,真空のH(+) -ATPアゼの16K成分に結合する
D J Goldstein1, M E Finbow, T Andresson
1Department of Pathology, Georgetown University, Washington, DC 20007.
Nature
|July 25, 1991
まとめ
牛のパピローマウイルス1型E5タンパク質は,真空のATPアゼ成分である16Kタンパク質に結合する. この陽子ポンプとの相互作用は,E5誘発の細胞変容中に観察された細胞の変化を説明する可能性がある.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- ウイルス学 ウイルス学 ウイルス学
背景:
- 牛のパピローマウイルス1型 (BPV-1) E5タンパク質は,重要な変容剤である.
- E5は内膜に局所化し,細胞タンパク質と相互作用する.
- E5は成長因子受容体のチロシンリン酸化を誘発する.
研究 の 目的:
- BPV-1 E5変換タンパク質に結合する細胞16Kタンパク質を識別する.
- 細胞変容におけるE5-16Kタンパク質相互作用の機能的意義を解明する.
主な方法:
- コイムノプレシピテーションは,相互作用するタンパク質を特定するための測定法です.
- 細胞コンパートメント内のタンパク質の局所化の分析.
- E5発現に反応する成長因子受容体のリン酸化の評価.
主要な成果:
- E5に関連した16Kタンパク質は,真空のATPases (v-ATPases) のサブユニットとして識別されました.
- この16K v-ATPase成分は,様々な水泡や膜複合体で見られる統合膜タンパク質です.
- 16K v-ATPaseタンパク質とのE5の相互作用は,成長因子受容体のリン酸化と相関しています.
結論:
- BPV-1 E5オンコタンパク質は,真空アテパースの16Kサブユニットと相互作用する.
- この相互作用は,エンドソームのコンパートメントの陽子ポンプの機能を妨害する可能性が高い.
- E5によるv-ATPase機能の破壊は,変換中に観察されたプレイオモルフな細胞変化の根底にある可能性があります.
関連する概念動画
02:33Initiation of Translation
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
01:51Mechanisms of Retrovirus-induced Cancers
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
01:30ATP Driven Pumps III: V-type Pumps
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
01:19Inhibitors of Virion Maturation and Assembly
As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...