Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Convergent Evolution01:54

Convergent Evolution

Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Gene Duplication and Divergence02:37

Gene Duplication and Divergence

The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
Introduction to Enzymes01:22

Introduction to Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

A biosynthetic survey of hypocrealean biocontrol fungi.

Nature chemical biology·2026
Same author

Expanding the scope of redox-balance growth coupling techniques with a carbon cofeeding strategy.

bioRxiv : the preprint server for biology·2026
Same author

Leveraging a synthetic biology approach to enhance BCG-mediated expansion of Vγ9Vδ2 T cells.

PloS one·2026
Same author

Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid.

ACS synthetic biology·2026
Same author

Synthetic biology for heterologous expression and engineering of fungal polyketide synthases.

Natural product reports·2026
Same author

Enzymology and Structural Basis of Glycosyltransferases Involved in Saponin C28 Carboxylic Acid <i>O</i>‑d‑Fucosylation.

JACS Au·2025
Same journal

Six ways to put the public at the heart of science and policy.

Nature·2026
Same journal

The complex truth about trust in science.

Nature·2026
Same journal

Have people stopped trusting science? The data tell a surprising story.

Nature·2026
Same journal

How FAIR data are helping to build trust in science.

Nature·2026
Same journal

Scientists should recognize their own political biases to build public trust.

Nature·2026
Same journal

Harmonizing standards and resources for the medical genome.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: May 11, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

酵素機能の異なる進化を設計した.

Yasuo Yoshikuni1, Thomas E Ferrin, Jay D Keasling

  • 1UCSF/UCB Joint Graduate Group in Bioengineering, Lawrence Berkeley National Laboratory, Berkeley, California 94710, USA.

Nature
|February 24, 2006
PubMed
まとめ
この要約は機械生成です。

研究者は,タンパク質の可塑性を理解することによって,新しい酵素を設計しました. この研究は,特製合成酵素の可塑性残留を改変し,特異的かつ活性な生物触媒を生成することによって,合理的な酵素設計を実証しています.

さらに関連する動画

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

関連する実験動画

Last Updated: May 11, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • 酵素工学とは

背景:

  • 乱交的な機能を持つタンパク質は,小さなアミノ酸の変化 (可塑性) を通して,より高い特異性と活性を発達させる.
  • この異なる分子進化の理解は,より特定で活性な酵素の設計を導くことができます.
  • 活性部位における可塑性残留は,機能的適応性にとって極めて重要です.

研究 の 目的:

  • プラスチックの残留が分子進化にどのように貢献するかを調査する.
  • 酵素工学のための合理的な設計方法論を策定する.
  • 変異した触媒機能を持つ新しいテルペン合成酵素を構築する.

主な方法:

  • 乱交性ガンマ-ヒュミュレン合成酵素の活性部位にプラスチック性残留物を探査した.
  • 数学的モデルを使用して,識別された可塑性残留物を体系的に再結合します.
  • 新型テルペン合成酵素を構成し,特徴づけました.

主要な成果:

  • 特定で活性な7つのテルペン合成酵素を成功裏に設計した.
  • これらの新しい酵素は,異なる反応経路を利用して,異なるセスクイテルペン製品を生成します.
  • プロテイン・スキャフォールドの進化性を活用する可能性を実証した.

結論:

  • 柔軟性に基づく合理的な酵素設計アプローチは効果的です.
  • この研究は,酵素工学における分子進化の理解の有用性に関する証拠を提供します.
  • 望ましい特異性および活動を持つカスタマイズされた酵素を作成する可能性を強調しています.