菌根性HLHタンパク質の機能的活性には,E12/E47のようなタンパク質とのヘテロオリゴメリゼーションが必要である
A B Lassar1, R L Davis, W E Wright
1Department of Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Cell
|July 26, 1991
まとめ
E12/E47のようなタンパク質は,ミオゲニンタンパク質MyoDとミオゲニン in vivoと相互作用する. この相互作用は,筋肉の分化と,下流の筋肉の遺伝子発現を調節するために重要である.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 発達生物学 発達生物学とは
背景:
- MyoDやmyogeninのようなMyogenicヘリックス・ループ・ヘリックス (HLH) タンパク質は,筋肉の分化の主な調節因子である.
- これらのタンパク質が関与する正確な相互作用と規制メカニズムは,筋肉の発達を理解するために重要です.
研究 の 目的:
- E12/E47のようなタンパク質とミオジェニックのHLHタンパク質 (MyoD, myogenin) の間のインビボ相互作用の証拠を提供すること.
- これらの相互作用が筋肉の遺伝子転写と分化に及ぼす機能的影響を明らかにする.
主な方法:
- COS細胞における共伝染測定は,トランスクリプションの相乗効果を評価するために行われます.
- タンパク質複合体を特定するために,特定の抗菌剤を用いた電泳運動移転シフトアッセイ (EMSA) を用いる.
- タンパク質のリン酸化状態の分析.
- 変化したE2A発現を持つ細胞における筋肉の分化評価.
主要な成果:
- MyoDとE47.7によるシネジスティックなトランスクリプション強化が実証された.
- MEF1部位でE12/E47のようなタンパク質と関連したMyoDまたはミオゲニンを含んだタンパク質複合体.
- E47関連がMyoDのリン酸化を変化させることを示した.
- 抑制されたE2A活性を持つ細胞における筋肉の微分化が減少していることが観察されました.
結論:
- E12/E47のようなタンパク質は,MyoDとミオゲニンと体内で物理的に相互作用する.
- これらの相互作用は,下流の筋肉調節体を活性化させ,末端の筋肉の分化を促進するために不可欠です.
- MyoDは,E12/E47のようなタンパク質との複合体で,筋肉の発達における中心的な調節点として作用する.
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