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Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

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In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

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Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

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Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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Updated: Jan 7, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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ウイルスの侵入:開いた芝生.

Mark Marsh1, Ari Helenius

  • 1Cell Biology Unit, MRC Laboratory for Molecular Cell Biology, and Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom. m.marsh@ucl.ac.uk

Cell
|February 25, 2006
PubMed
まとめ
この要約は機械生成です。

ウイルスの複製と宿主相互作用を理解することは,抗ウイルス戦略の開発の鍵です. 新しい研究は,ウイルスが宿主細胞にどのように侵入するかを明らかにし,新しい治療標的を明らかにしています.

さらに関連する動画

Primary Culture of Mouse Dopaminergic Neurons
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
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関連する実験動画

Last Updated: Jan 7, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

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Published on: January 20, 2015

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11:58

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科学分野:

  • ウイルス学 ウイルス学 ウイルス学
  • 細胞生物学 細胞生物学
  • システム生物学 システム生物学

背景:

  • 効果的な抗ウイルス戦略を開発するには,ウイルスの複製サイクルと宿主相互作用の詳細な知識が必要です.
  • 動物ウイルスは,宿主細胞に入り込むためにさまざまな経路を利用し,その生命周期における重要なステップです.

研究 の 目的:

  • 宿主細胞にウイルスが侵入する複雑なメカニズムを解明する.
  • 抗ウイルス介入のための新しい治療標的を特定する.

主な方法:

  • 細胞生物学のテクニック
  • 生きている細胞のイメージングです.
  • システム生物学のアプローチは,システム生物学のアプローチです.

主要な成果:

  • 動物ウイルスが宿主細胞への侵入に利用する複数の経路についての詳細な洞察.
  • ウイルス感染症におけるエンドサイトーシスと細胞内膀輸送の役割の説明.
  • ウイルスの複製を阻害する新たな標的を特定する.

結論:

  • ウイルスの侵入メカニズムを理解することで,宿主と病原体の相互作用に関する知識が大幅に進歩します.
  • 新しい治療戦略は,ウイルスの感染の初期段階,特にウイルスの侵入をターゲットにすることで開発することができます.
  • 細胞生物学とシステム生物学における継続的な研究は,ウイルス性疾患との闘いにおいて極めて重要です.