細胞のレチノール結合タンパク質II型遺伝子の直接的な繰り返しは,RXRとRARによる差異的調節を与える
D J Mangelsdorf1, K Umesono, S A Kliewer
1Howard Hughes Medical Institute, La Jolla, California 92186-5800.
Cell
|August 9, 1991
まとめ
レチノ酸は,レチノ酸受容体 (RAR) ではなく,レチノイドX受容体 (RXR) 経由で細胞のレチノール結合タンパク質II型 (CRBPII) 遺伝子発現を上調する. これは,新しいRX応答要素を特定し,ビタミンAの代謝経路を明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 栄養バイオケミストリー
- 遺伝学 遺伝学とは
背景:
- レチノ酸は,ビタミンAの誘導体であり,核受容体:レチノ酸受容体 (RAR) とレチノイドX受容体 (RXR) を介して遺伝子発現を調節します.
- 細胞レチノール結合タンパク質II型 (CRBPII) は,腸内ビタミンAの吸収に不可欠です.
研究 の 目的:
- レチノ酸によるCRBPII遺伝子発現の上昇調節を媒介する特定の核受容体 (RARまたはRXR) を調査する.
- この転写の調節に責任を負うDNA配列を特定するために.
主な方法:
- レチノ酸に対する反応におけるCRBPII遺伝子発現の分析,受容体の存在が変化する.
- CRBPIIプロモーター内のシス作用要素の識別と特徴付け.
主要な成果:
- レチノ酸は,特にRXRの存在下でのCRBPII遺伝子発現を著しく上調しますが,RARではありません.
- AGGTCAのタンデムリピートによって特徴づけられる新しいRX応答要素が,CRBPIIプロモーターで特定されました.
結論:
- この発見は,CRBPII経由でビタミンAの代謝を調節するためのRXR媒介経路を示しています.
- この発見は,RARとRXRの信号伝達経路を区別する方法を提供します.
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