ネズミのエコトロピックレトロウイルス受容体によるカチオンアミノ酸の輸送
J W Kim1, E I Closs, L M Albritton
1Howard Hughes Medical Institute, Boston, Massachusetts.
Nature
|August 22, 1991
まとめ
マウリン白血病ウイルス (MuLV) 受容体は,アミノ酸輸送体として識別されます. この発見は,これらのウイルス受容体の新しい細胞機能を明らかにし,細胞生物学とウイルス学に影響を与えています.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- エコトロピクマウリン白血病ウイルス (MuLV) に対する感受性は,特定の膜受容体に依存します.
- 感染した細胞は封筒タンパク質 (gp70) を生成し,この受容体に結合し,さらなる感染を阻止します.
- MuLV受容体タンパク質の細胞機能は,ほとんど不明のままである.
研究 の 目的:
- MuLV受容体タンパク質の細胞機能を調べるために.
- MuLV受容体と他の既知のタンパク質との間の潜在的な類似性を特定する.
- MuLV受容体-ウイルス結合の機能的影響を調査する.
主な方法:
- GenBankデータベースに対するMuLV受容体のコンピュータベースの配列比較.
- MuLV受容体および関連するタンパク質の膜横断ドメインの分析.
- メッセンジャーRNAの注射後の受容体機能を研究するためのXenopus卵細胞発現システム.
主要な成果:
- MuLV受容体は,その膜を横断する領域において,Saccharomyces cerevisiaeからのアルギニンおよびヒスティディン浸透剤と構造的類似性を共有しています.
- MuLV受容体を発現するXenopus卵細胞は,カチオン性アミノ酸 (L-アルギニン,L-リシン,L-オルニチン) の吸収が増加したことを示した.
- MuLV受容体の輸送特性と発現パターンは,哺乳類のカチオンのアミノ酸トランスポーター,y+の既知の性質と一致しています.
結論:
- MuLV受容体タンパク質は,カチオンのアミノ酸のトランスポーターとして機能します.
- この発見は,ウイルスの受容体にとって新しい細胞の役割を提供する.
- この研究は,ウイルスの侵入メカニズムを基本的な細胞輸送プロセスと関連付けています.
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